Graph pad prism 4.0b software

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GraphPad Prism 4.0b is a data analysis and graphing software. It provides tools for data analysis, curve fitting, and creating publication-quality graphs.

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2 protocols using graph pad prism 4.0b software

Glucose Uptake Assay in Leishmania

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Glucose uptake assays employed logarithmic phase L. mexicana promastigotes of the Δlmxgt1-3 hexose transporter null mutant strain expressing either PfHT or GLUT1 from an episomal expression vector [17 (link)]. Parasites (1x10⁷) were resuspended in 100 μL phosphate buffered saline, pH 7.4. Prior to uptake, parasites were pre-incubated for 5 min with inhibitor, and uptake was initiated by adding 100 μL of 200 μM [³H] D-glucose diluted to 4.0 μC_i/mL. Uptake was terminated, typically after 1 min, by centrifuging the parasites through a layer of dibutyl phthalate, as described [23 (link)]. Control uptake assays employing [³H] hypoxanthine, [³H] uridine, and [³H] L-proline were performed similarly. Analysis of kinetic data was performed using Graph Pad Prism 4.0b software (Graph Pad).

Ortiz D., Guiguemde W.A., Johnson A., Elya C., Anderson J., Clark J., Connelly M., Yang L., Min J., Sato Y., Guy R.K, & Landfear S.M. (2015). Identification of Selective Inhibitors of the Plasmodium falciparum Hexose Transporter PfHT by Screening Focused Libraries of Anti-Malarial Compounds. PLoS ONE, 10(4), e0123598.

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Statistical Analysis of Tumor Growth and Patient Data

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All data (means ± standard error of mean [xenografts] or standard deviation [in vitro]) were analysed using Excel or GraphPad Prism 5. For tumour growth analysis, a parametric generalised linear model was performed using GraphPad Prism 4.0b software. For western blot analysis, cell proliferation and qRT-PCR, statistical significance was determined using the Kruskal-Wallis one-way analysis of variance. All in vitro experiments were replicated on at least three occasions. For patient data, the χ² test was used to determine association between PAT4 expression and categorical clinical variables (Supplementary Table S1). Cox-regression analysis was employed to determine prognostic factors in univariate (Supplementary Table S2) and multivariate survival models (Supplementary Table S3). The log rank (Mantel-Cox) test was used to assess the significance of differences in relapse-free survival between Kaplan-Meier curves. Statistical analyses were performed using SPSS Statistics (Version 21.0).

Fan S.J., Snell C., Turley H., Li J.L., McCormick R., Perera S.M., Heublein S., Kazi S., Azad A., Wilson C., Harris A.L, & Goberdhan D.C. (2015). PAT4 levels control amino-acid sensitivity of rapamycin-resistant mTORC1 from the Golgi and affect clinical outcome in colorectal cancer. Oncogene, 35(23), 3004-3015.

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