Two M. smegmatis strains, differing in the levels of the mmpS5-mmpL5 operon expression, were used in the study: the wild type (w.t.) M. smegmatis mc2 155, with a basal level of mmpS5-mmpL5 expression, and M. smegmatis atr9c—a recombinant strain with a mutation in MSMEG_1380 gene (insC8), leading to over-expression of the mmpS5-mmpL5 operon [14 (link)]. M. smegmatis were cultured in liquid Middlebrook 7H9 medium (Himedia, Mumbai, India) supplemented with oleic albumin dextrose catalase (OADC, Himedia, Mumbai, India), 0.1% Tween-80 (v/v), and 0.4% glycerol (v/v) in the Multitron incubator shaker (Infors HT, Basel, Switzerland) at 37 °C and 250 rpm, while soyabean-casein digest agar (M290, Himedia, Mumbai, India) was used as the solid media, with incubation at 37 °C.
Soyabean casein digest agar
Manufactured by HiMedia
Sourced in India
Soyabean-casein digest agar is a general-purpose microbiological culture medium. It supports the growth of a wide range of microorganisms, including bacteria and fungi. The medium is composed of soy peptone, casein peptone, sodium chloride, and agar, which provide nutrients and a solidifying agent for cultivating microbial colonies.
Automatically generated - may contain errors
Lab products found in correlation
Middlebrook 7h9 medium, by HiMedia (1 mentions)
Multitron incubator shaker, by Infors (1 mentions)
Yeast extract, by Hardy Diagnostics (1 mentions)
Sabouraud dextrose agar, by HiMedia (1 mentions)
Tryptic soya broth, by HiMedia (1 mentions)
Epigallocatechin, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Hyaluronic acid sodium salt from rooster comb, by Merck Group (1 mentions)
Clindamycin, by Thermo Fisher Scientific (1 mentions)
N 3 2 furyl acryioyl leu gly pro ala, by Merck Group (1 mentions)
3 4 dihydroxy l phenylalanine l dopa, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Syringic acid, by Merck Group (1 mentions)
3 protocols using soyabean casein digest agar
1
Comparative Analysis of M. smegmatis Strains
2
Characterization of Antimicrobial Agents
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In this study, all general culture media were obtained from India, Soyabean Casein Digest Agar (TSA), Sabouraud Dextrose Agar (SDA) and Tryptic Soya Broth (TSB) (HiMedia, Mumbai, India). Methyl paraben (MP) (Hangzhou Zhongbao Imp&Exp Corp. LTD, Hangzhou, China) and propyl paraben (PP) (Elementz, Mumbai, INDIA), Sodium benzoate (SB) (Snowhite Chemical Company Limited, Nanjing, China), polysorbate 80 (Elementz, Mumbai, INDIA), cetomacrogol 1000 (BASF, Düsseldorf, Germany) and polyoxyl 40 hydrogenated castor oil (Spak Orgachem, Mumbai, India) were kindly donated by (Ibn Al-Haytham Pharmaceutical Industries Co., Aleppo, Syria). Lecithin (HENRY FRANCE S.A.S, Lyon airport, France), yeast extract (HARDY DIAGNOSTICS, California, United States), sodium thiosulphate and sodium thioglycolate (Sisco Research Laboratories Pvt. Ltd. (SRL), Mumbai, India) were taken from the chemistry laboratories of the faculty of Pharmacy, University of Aleppo. Ibuprofen commercial preparation (Universal Pharmaceutical Industries UNIPHARMA, Damascus, Syria) and Cefpodoxime proxetil commercial preparation (Alpha Pharmaceutical Industries Company, Aleppo, Syria).
3
Colorimetric Assay for Enzyme Activities
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Gallic acid, collagenase activity colorimetric assay kits, elastase from porcine pancreas, N-succinyl-(Ala)-3-nitroaniline (SANA), N-[3-(2-furyl) acryIoyl]-Leu-Gly-Pro-Ala (FALGPA), hyaluronidase from bovine testes, 3′,4′-dihydroxy-l-phenylalanine (L-DOPA), tyrosinase from lyophilized mushroom powder, syringic acid, and epigallocatechin were purchased from Sigma-Aldrich ® , USA. Hyaluronic acid sodium salt from rooster comb from Sigma-Aldrich ® (China), ascorbic acid and 2,2-diphenyl-1-picrylhydrazy (DPPH) from Sigma-Aldrich ® (Germany), folin ciocalteu reagents from Loba (India), and oleanolic acid from Chengdu Biopurify Phytochemicals (China) were used. Mueller Hinton broth (MHB), Mueller Hinton agar (MHA), and soyabean casein digest agar were procured from Himedia, India. Clindamycin was obtained from Oxoid, UK. Bovine serum albumin was purchased from Acros Organics, USA. All chemicals for formulation were cosmeceutical grade and purchased from Chemipan Corporation Co., Ltd (Thailand). Other solvents and reagents used were of analytical grade.
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