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3 protocols using isopropanol

1

Biochemical Assays for Lipid Metabolism

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Curcumin and piperine were purchased from Sigma-Aldrich (St. Louis, MO, USA). TC, triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C) and total bile acid (TBA) kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, Jiangsu, China). The free cholesterol (FC) kit was purchased from Applygen Technologies Inc. (Beijing, China). Apolipoprotein AI (ApoAI) and ApoB kits were purchased from Shanghai Rongsheng Biotech Co., Ltd. (Shanghai, China). The bicinchoninic acid assay kit was obtained from Tiangen Biotech Co., Ltd. (Beijing, China). Ethanol and isopropanol of analytical grade were purchased from Dikma Technologies, Inc. (Beijing, China). TRIzol® reagent was purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). The RevertAid™ First Strand cDNA Synthesis kit and the Maxima™ SYBR-Green/Fluorescein qPCR Master mix (2X) were purchased from Thermo Fisher Scientific (Burlington, ON, Canada).
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2

Analysis of Sudan Dye Compounds

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Sudan I, Sudan II, Sudan III, Sudan IV, Sudan red 7B, Sudan red G, Sudan red B, and Para red were obtained from Dr. Ehrenstorfer GmbH (Augsburg, Germany). Water was prepared by a Milli-Q ultrapure water system (Millipore, Bedford, MA.). HPLC-grade acetonitrile, methanol, dichloromethane, and isopropanol were purchased from Dikma (Lake Forest, CA). Formic acid was obtained from Acros Organics (Morris Plains, NJ).
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3

Comprehensive Analytical Protocol for Psychoactive Drugs

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Rhodamine B, risperidone-d 4 (100 μg mL -1 in Methanol) and aripiprazole-d 8 (100 μg mL -1 in acetonitrile) were provided from Sigma-Aldrich (Shanghai, China). Aripiprazole, dehydroaripiprazole and risperidone were provided from J&K Scientific (Beijing, China). Formic acid, trifluoroacetic acid, pyrrole, cetyltrimethylammonium bromide (CTAB) and graphene oxide (1 mg mL -1 , dispersion in water) were provided from Aladdin Bio-Chem (Shanghai, China). Methanol, acetonitrile and isopropanol of HPLC grade were provided from Dikma (Beijing, China). Phosphate buffered saline (pH 7.2-7.4, 0.01 M) was provided from Solarbio Life Science (Beijing, China). Artificial urine was provided from Phygene (Fuzhou, China). Pure water was obtained from a Millipore water purification system.
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