For DXA analysis, eight-week-old male Imprinting Control Region (ICR) mice (n = 22) were purchased from Orient Bio Inc. (Seoul, Korea). Twelve mice were caged together in groups of four and the remaining was caged individually for this study. Three cages were prepared for group caging (n = 4 per cage) and ten cages were prepared for individual caging (n = 1 per cage). For open field test, eight-week-old male Imprinting Control Region (ICR) mice (n = 20) were purchased from Orient Bio Inc. (Seoul, Korea) and caged under the same conditions as before. Sixteen mice were caged together in groups of four and the remaining was caged individually. All mice were caged in the same size cages (260 (W) × 420 (D) × 180 (H) mm) for four weeks under regulated conditions with 12-hour light-dark cycles and food and water were provided ad libitum. The animal experiments were approved by the Committee for the Care and Use of Laboratory Animals at Yonsei University and performed in accordance with the National Institutes of Health guide for the care and use of laboratory animals.
Imprinting control region mice
Manufactured by Orient Bio
Imprinting Control Region (ICR) mice are a specific strain of laboratory mice used in research. ICR mice are characterized by the presence of a genetic imprinting control region, which regulates the expression of certain genes. This strain is commonly used in studies related to genomic imprinting, embryonic development, and other research areas where the control of gene expression is of interest.
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Lab products found in correlation
Trap stain kit, by Merck Group (1 mentions)
Cell culture media and supplements, by Thermo Fisher Scientific (1 mentions)
Rnazol, by Takara Bio (1 mentions)
Soluble recombinant mouse rankl srankl, by Thermo Fisher Scientific (1 mentions)
Phospho jnk thr183 tyr185, by Cell Signaling Technology (1 mentions)
P38 mapk, by Cell Signaling Technology (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
2 protocols using imprinting control region mice
1
DXA and Open Field Analysis of ICR Mice
2
Osteoclastogenesis Regulation by Poncirin
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Cell culture media and supplements were purchased from Invitrogen (Carlsbad, CA, USA). Soluble recombinant mouse RANKL (sRANKL) was purchased from Peprotech (NJ, USA). RNAzol and all PCR reagents were obtained from Takara Bio Inc (Shiga, Japan). TRAP stain kit was from Sigma-Aldrich (St. Louis, MO, USA). Antibodies for p38 MAPK, ERK, JNK, phospho-p38MAPK (Thr180/Tyr182), phospho-ERK, phospho-JNK (Thr183/Tyr185), and anti-β-actin were procured from Cell Signaling Technology (Danvers, MA, USA). Poncirin was provided by the Korea Food and Drug Administration (KFDA, Ochang, Korea). Imprinting Control Region (ICR) mice were purchased from ORIENT Bio (Seongnam, Korea).
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