Antibody against vdac1

DMEM‐F12 (Dulbecco's modified Eagle's medium‐F12) was purchased from Sigma (Sigma‐Aldrich). FBS (foetal bovine serum), ITS (insulin‐transferrin‐selenium), L‐glutamine, DMSO (dimethyl sulfoxide), MTT (3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazoliumbromide), penicillin, streptomycin and sirtinol were purchased from Sigma (Sigma‐Aldrich). Antibodies against sirt1, ERα, IGF1R, CCND1 (cyclin D1), vimentin, N‐cadherin, bax (bcl2 associated x), bcl‐2 (B‐cell lymphoma 2), parp‐1 (poly(ADP‐ribose) polymerase 1), cytochrome c, pCREB‐1^Ser133 (phospho cyclic AMP response element binding) and GAPDH (glyceraldehyde 3‐phosphate dehydrogenase) were purchased from Santa Cruz (Santa Cruz Biotechnology, Inc, Heidelberg, Germany). Antibody against VDAC1 (voltage‐dependent anion‐selective channel protein 1)/porin) was purchased from Abcam (Abcam, Cambridge, UK). HRP (Horseradish peroxidase)‐conjugated secondary antibodies were purchased from Bethyl (Bethyl Laboratories, Montgomery, TX, USA) and ECL (Electrochemiluminescence) Western blotting detection system from Santa Cruz (Santa Cruz Biotechnology, Santa Cruz CA, USA). The TRIzol reagent, High Capacity cDNA Reverse Transcription Kit and PowerUp™ SYBR™ Green Master Mix were purchased from Thermo Fisher Scientific (Thermo Fisher Scientific).

Culture media for ECs were purchased from Lonza (Houston, TX, USA). Antibody against NAXE was obtained from Novus Biologicals (Littleton, CO, USA). Antibodies against GAPDH and Lamin A/C were obtained from Cell Signaling Technology (Danvers, MA, USA). Antibody against VDAC1 was obtained from Abcam (Boston, MA, USA). Peroxidase-labeled second antibodies were obtained from Jackson ImmunoResearch (Buffalo, NY, USA). NAXE short interference RNA (siRNA) and scrambled control siRNA were purchased from Life Technologies (Carlsbad, CA, USA). The Seahorse XF Cell Mito Stress Test Kit was purchased from Agilent (Santa Clara, CA, USA). Common chemical reagents were purchased from Sigma Aldrich (St. Louis, MO, USA). Reagents for sample preparation and primary metabolite analysis by mass spectrometry included acetonitrile, LCMS grade (JT Baker; Cat. No. 9829-02), isopropanol, HPLC grade (JT Baker; Cat. No. 9095-02), crushed ice pH paper 5-10 (EMD Chem. Inc. Gibbstown, NJ, USA), and nitrogen line with pipette tip 18 MΩ pure water (Millipore, Burlington, MA, USA).