50 ml screw cap tube

Twelve patients diagnosed with Glioblastoma multiforme (Glioblastoma, IDH-wild type) according to the WHO classification [57 (link)] were included in the study. The study design was approved by PV6041 by the Ethical Commission of the Hamburg Medical Chamber (Ethik-Kommission der Ärztekammer, Hamburg, Germany).
Tumor operations were performed at the Asklepios Klinik Nord-Heidberg (Hamburg, Germany). For cell differentiation during surgery, tumor cells were stained with 5-aminolevulinic acid. After surgery, tissue samples were placed in sterile screw cap tubes with a standard cap (2 mL, Type H, Sarstedt, Nümbrecht, Germany) previously filled with 1.5 mL DMEM supplemented with 10% fetal bovine serum (FBS) (PAN-Biotech, Aidenbach, Germany). Tissue samples were collected from four different tumor regions, and each was placed separately in one of the reaction tubes. The tubes were placed in a 50 mL screw cap tube (Sarstedt, Nümbrecht, Germany) to avoid any contamination during transport. The tissue samples were immediately transported to the cell culture laboratory at the Bernhard Nocht Institute for Tropical Medicine (Hamburg, Germany) to start the cell cultivation procedure immediately.

S. oralis strain ATCC 9811 was obtained from the American Type Culture Collection (ATCC) and P. gingivalis strain ATCC 33277/DSM 20709 from the German Collection of Microorganisms and Cell Cultures (DSMZ). The strains were grown under anaerobic conditions (80% N₂, 10% H₂, 10% CO₂; anaerobic workbench Concept 400-M, Ruskinn Technology Ltd., Pencoed, UK) at 37 °C for 24 h in 50 mL screw cap tube (Sarstedt, Nümbrecht, Germany) with Brain Heart Infusion Medium (BHI; Oxoid, Wesel, Germany) containing 10 µg/mL vitamin K (Roth, Karlsruhe, Germany; BHI/VitK) to obtain 24-h-old precultures for further processing.