Tbx medium tryptone bile x gluc

Manufactured by Liofilchem

Sourced in Italy

TBX Medium (Tryptone Bile X-Gluc) is a selective and differential culture medium used for the isolation and enumeration of Escherichia coli and other coliform bacteria. It contains tryptone, bile salts, and the chromogenic substrate X-Gluc, which allows for the detection of beta-glucuronidase activity, a characteristic of E. coli.

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Lab products found in correlation

Slanetz bartley agar base, by Liofilchem (3 mentions) Pseudomonas cn agar, by Liofilchem (2 mentions)

Spelling variants of this product

Tryptone Bile X-Gluc (2 citations) Tryptone (2 citations)

5 protocols using tbx medium tryptone bile x gluc

Microbial Growth Impact of Hybrid Materials

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In order to study the effect of the samples on the microbial growth, E. coli (ATCC 25922) and P. aeruginosa (ATCC 10145) were used. E. coli was cultured in TBX Medium (Tryptone Bile X-Gluc) (Liofilchem, Roseto degli Abruzzi, Italy), while P. aeruginosa in Pseudomonas CN Agar (Liofilchem, Roseto degli Abruzzi, Italy).
Afterwards, the bacterial cultures were diluted in distilled water to produce a bacterial cell suspension of 10 × 10⁵ CFU/mL. Both bacteria were inoculated in absence and in presence of the hybrid materials. E. coli was incubated with the materials for 24 h at 44 °C, while the P. aeruginosa for 48 h at 36 °C. The microbial growth was evaluated by observing the diameter of the inhibition halo (ID). The obtained values are the mean standard (SD) deviation of measurements carried out on samples analyzed three times.

Catauro M., Tranquillo E., Barrino F., Blanco I., Dal Poggetto F, & Naviglio D. (2018). Drug Release of Hybrid Materials Containing Fe(II)Citrate Synthesized by Sol-Gel Technique. Materials, 11(11), 2270.

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Antibacterial Efficacy of Hybrid Materials

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Antibacterial properties of synthesized hybrid materials were evaluated against the Gram-negative Escherichia coli (ATCC 25922) and Gram-positive Enterococcus faecalis (ATCC 9212) bacteria. To this purpose, the bacterial culture was diluted in distilled water to produce a bacterial cell suspension of 10 × 10⁵ colony-forming unit (CFU)/mL. E. coli was inoculated in TBX Medium (Tryptone Bile X-Gluc) (Liofilchem, Roseto degli Abruzzi (TE), Italy), while E. faecalis was inoculated in Slanetz Bartley Agar Base (Liofilchem, Roseto degli Abruzzi (TE), Italy) in the presence of 100 mg of the hybrid powders. Afterward, the bacteria were incubated with the materials for 24 h at 44 °C and 48 h at 36 °C, respectively. The microbial growth was evaluated by observing the diameter of the inhibition halo (ID). The obtained values were expressed as mean ± standard deviation (SD) values of the measurements carried out in triplicate [24 (link)].

Catauro M., Barrino F., Dal Poggetto G., Crescente G., Piccolella S, & Pacifico S. (2020). New SiO2/Caffeic Acid Hybrid Materials: Synthesis, Spectroscopic Characterization, and Bioactivity. Materials, 13(2), 394.

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Antibacterial Properties of SiO2/PEG/CGA Hybrids

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Escherichia coli, Gram-negative, (ATCC 25922) and Enterococcus faecalis, Gram-positive (ATCC 29212), were used to study antibacterial properties of SiO₂/PEG/CGA hybrids in function of different amounts of CGA [18 (link)].
The bacterial culture was diluted in distilled water to produce a bacterial cell suspension of 10×10⁵ CFU/mL. E. coli was inoculated in TBX Medium (Tryptone Bile X-Gluc) (Liofilchem, Roseto degli Abruzzi (Te), Italy), while, E. faecalis was inoculated in Slanetz Bartley Agar Base (Liofilchem, Italy) in presence of 100 mg of the hybrid powders. Afterward, the bacteria were incubated with the materials for 24 h at 44 °C and 48 h at 36 °C, respectively. The microbial growth was evaluated by observing the diameter of the inhibition halo (ID). The obtained values are the mean standard (SD) deviation of the measurements carried out on samples analyzed three times.

Catauro M., Barrino F., Dal Poggetto G., Crescente G., Piccolella S, & Pacifico S. (2019). Chlorogenic Acid Entrapped in Hybrid Materials with High PEG Content: A Strategy to Obtain Antioxidant Functionalized Biomaterials?. Materials, 12(1), 148.

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Microbial Growth Influenced by Materials

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The influence of materials on the microbial growth of Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 10145) was assessed.
E. coli was incubated in TBX medium (tryptone bile X-gluc) (Liofilchem, Italy), while Pseudomonas aeruginosa in Pseudomonas CN agar (Liofilchem, Italy). The bacterial cell suspension of 10 × 10⁵ cfu/mL was obtained by dilution in distilled water. Afterwards, E. coli was incubated in presence of the different materials for 24 h at 44 °C, while the Pseudomonas aeruginosa was incubated for 48 h at 36 °C.
The diameter of the inhibition halo (ID) was measured to evaluate microbial growth. The results were obtained on samples analyzed three times and used to determine the mean standard (SD) deviation of measurements.

Tranquillo E., Barrino F., Dal Poggetto G, & Blanco I. (2019). Sol–Gel Synthesis of Silica-Based Materials with Different Percentages of PEG or PCL and High Chlorogenic Acid Content. Materials, 12(1), 155.

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Evaluating Antibacterial Properties of Geopolymers

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In order to estimate the antibacterial properties of the geopolymers, Escherichia coli, (ATCC 25922) from Gram-negative family, and Enterococcus faecalis, (ATCC 29212) from Gram-positive family, were grown in the absence and presence of the synthesized materials extracted after 7, 14 and 28 curing days at room temperature. Samples used for the analyses were finely ground and pressed to obtain disks of 100 and 200 mg of weight that were radiated by UV light for 1 h for sterilization. The bacterial suspension of 10⁵ CFU/mL was obtained by diluting the strains in distilled saline water (0.9% NaCl). After plating E. coli in TBX Medium (Tryptone Bile X-Gluc) (Liofilchem, Italy) and E. faecalis in Slanetz Bartley agar base (Liofilchem, Italy), the samples were placed in the center of Petri dishes. E. coli and E. faecalis dishes were incubated at 44 °C for 24 h and 36 °C for 48 h, respectively. The diameter of inhibition halos (IDs) in relation to Petri dish diameter (DD) (6 cm) was measured (Figure S2). Four measures for each sample were carried out in to determine the mean Standard Deviation. Results are expressed as Bacterial Viability (in percentage) = [(DD-IDs)/(DD)] × 100. Bacterial viability in the control plate, i.e., without geopolymeric samples, is expressed as 100%. The mean Standard Deviation is expressed as Relative Standard Deviation (RSD).

Dal Poggetto G., Catauro M., Crescente G, & Leonelli C. (2021). Efficient Addition of Waste Glass in MK-Based Geopolymers: Microstructure, Antibacterial and Cytotoxicity Investigation. Polymers, 13(9), 1493.

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