Hepg2 and hela cells

Manufactured by American Type Culture Collection

Sourced in Switzerland, United States

HepG2 and HeLa cells are established cell lines commonly used in scientific research. HepG2 cells are derived from a human hepatocellular carcinoma, while HeLa cells are derived from a human cervical carcinoma. These cell lines provide standardized and renewable sources of cells for in vitro experiments and assays.

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HepG2 (2339 citations) HeLa cells (967 citations) HepG2 cells (680 citations)

5 protocols using hepg2 and hela cells

Culturing Retinoblastoma and Other Cell Lines

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The HXO-Rb44 retinoblastoma cells, ARPE-19, and HepG2 and HeLa cells were all purchased from the ATCC. All the cells were cultured with the DMEM culture medium in the standard condition of 37°C, 5% CO₂.

Zhang J., Liu Z.N, & Deng G.H. (2021). Anticancer Activity of New Na(I) Complex on Retinoblastoma Cells via Inhibiting PI3K/AKT/mTOR Pathway. Journal of Oncology, 2021, 9403333.

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Nanodiamonds for Cellular Assays

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All materials were acquired from Life Technologies (Carlsbad, CA, USA) unless otherwise specified. Detonation nanodiamonds were purchased from the Nanocarbon Research Institute (Nagano, Japan). High pressure high temperature (HPHT) nanodiamonds average size 45 nm contained about 100–120 ppm nitrogen, i.e., starting material for preparation of fluorescent nanodiamond, were sourced from Microdiamant AG, Switzerland. HepG2 and HeLa cells were acquired from ATCC (Manassas, VA, USA). Cell culture media and supplements were acquired from VWR (Radnor, PA, USA). Caspase Glo-3/7 and Cytotox 96 were acquired from Promega Corporation (Madison, WI, USA). Daunorubicin was acquired from Sigma-Aldrich (St. Louis, MO, USA).

Moore L., Grobárová V., Shen H., Man H.B., Míčová J., Ledvina M., Štursa J., Nesladek M., Fišerová A, & Ho D. (2014). Comprehensive Interrogation of the Cellular Response to Fluorescent, Detonation and Functionalized Nanodiamonds. Nanoscale, 6(20), 11712-11721.

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Overexpression and Enzyme Assays

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Mammalian overexpression plasmids and siRNA were purchased from Origene (Rockville, MD). HepG2 and HeLa cells were obtained from ATCC (Manassas, VA) and maintained using accompanying protocols. Cell culture materials and lipofection reagents were purchased from ThermoFisher (Waltham, MA). Buffer salts were acquired from Sigma-Aldrich (St. Louis, MO) as well as methyltransferase probe substrates unless otherwise indicated. AdoMet and molecular biology kits, including the Q5 Site-Directed Mutagenesis Kit, were obtained from New England Biolabs (Ipswich, MA). Stellar competent cells were purchased from Takara (Mountain View, CA). LOBSTR-BL21(DE3) competent cells were bought from Kerafast (Boston, MA). CHAPS detergent and UPLC-grade solvents were obtained from Fisher Scientific (Hampton, NH). Sequencing grade porcine trypsin and MTaseGlo Methyltransferase Assays were purchased from Promega (Madison, WI). 1,2-Dimyristoyl-sn-glycero-3-PG (DMPG) was obtained from Cayman Chemical (Ann Arbor, MI). The active metabolite of prasugrel was a gift from Dr. Allan Rettie (University of Washington).

Maldonato B.J., Russell D.A, & Totah R.A. (2021). Human METTL7B is an alkyl thiol methyltransferase that metabolizes hydrogen sulfide and captopril. Scientific Reports, 11, 4857.

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Transposition Assay in HeLa and HepG2 Cells

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HeLa and HepG2 cells (American Type Culture Collection, Manassas, VA, USA) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplied with 10% foetal bovine serum and 1% penicillin–streptomycin at 37^o under 5% CO₂ in humidified air. For transposition assays, the cells were planted on six-well plates at 3 × 10⁵ /well the day before transfection. 1 μg DNA (donor plasmid and helper plasmid at 1:1 ratio) with 2 μL transfection reagent of Transal-LT1 (Mirus Bio LLC, Madison, WI, USA) was applied for each well. The empty modified vector pcDNA3.0 was used to fill up in negative controls. At 48 h post-transfection, cells were replated on 10 cm dishes and trypsinized (10% or 1% plating). After selection with G418 (1 mg/mL for HeLa cells) for 14 days, cells were 4% paraformaldehyde-fixed, 0.2% methylene blue-stained and blue colonies-counted.

Gao B., Zong W., Miskey C., Ullah N., Diaby M., Chen C., Wang X., Ivics Z, & Song C. (2020). Intruder (DD38E), a recently evolved sibling family of DD34E/Tc1 transposons in animals. Mobile DNA, 11, 32.

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Cell Culture Protocol for HeLa and HepG2

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HeLa and HepG2 cells (American Type Culture Collection) were cultured in DMEM medium (Gibco, catalog no. 11995065) supplemented with 10% (v/v) fetal bovine serum (Gibco, catalog no. 16000044), 100 U/mL of penicillin and 100 μg/mL streptomycin (Gibco, catalog no. 15140122) at 37°C with 5% CO₂.

Pajdzik K., Lyu R., Dou X., Ye C., Zhang L.S., Dai Q, & He C. (2024). Chemical manipulation of m1A mediates its detection in human tRNA. RNA, 30(5), 548-559.

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