All chemicals were used as supplied by vendors without further purification. Imidazole, Invitrogen Novex WedgeWell 14% Tris-Glycine Mini Protein Gels, Isopropy-ß-D-thiogalactopyranoside (IPTG), lysozyme, NativePAGE 4 to 16% Bis-Tris Mini Protein Gels, NativeMark Unstained Protein Standard, Spectra Multicolor Broad Range Protein Ladder, Thermo Scientific Pierce 660 nm Protein Assay Reagent, Tris base, Tris HCl, all restriction enzymes, and all cell culture media and reagents were purchased from Fisher Scientific, Inc. (USA). Gibson Assembly Master Mix was purchased from NEB (USA). Amicon Ultra 0.5 mL centrifugal units and Benzonase nuclease were purchased from MilliporeSigma (USA). BL21 (DE3) Electrocompetent Cells used for Escherichia coli expression were also purchased from MilliporeSigma (USA). Bis-tris propane from Research Products International (USA) was used for the assembly buffer. Ni-NTA agarose from Gold Biotechnology, Inc. (USA) was used for His-tagged protein purification.
Novex wedgewell 14 tris glycine mini protein gels
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Novex WedgeWell 14% Tris-Glycine Mini Protein Gels are a laboratory product designed for protein electrophoresis. The gels are pre-cast and have a 14% acrylamide concentration, utilizing a Tris-Glycine buffer system. The gels are offered in a mini format suitable for use in standard electrophoresis equipment.
Automatically generated - may contain errors
Lab products found in correlation
Tris base, by Thermo Fisher Scientific (2 mentions)
Gibson assembly master mix, by New England Biolabs (2 mentions)
Benzonase nuclease, by Merck Group (2 mentions)
Amicon ultra 0.5 ml centrifugal units, by Merck Group (2 mentions)
Lysozyme, by Thermo Fisher Scientific (1 mentions)
Nativemark unstained protein standard, by Thermo Fisher Scientific (1 mentions)
Spectra multicolor broad range protein ladder, by Thermo Fisher Scientific (1 mentions)
Ni nta agarose, by GoldBio (1 mentions)
Pierce 660 nm protein assay reagent, by Thermo Fisher Scientific (1 mentions)
Nativepage 4 to 16 bis tris mini protein gels, by Thermo Fisher Scientific (1 mentions)
Tris hcl, by Thermo Fisher Scientific (1 mentions)
Imidazole, by Thermo Fisher Scientific (1 mentions)
Q5 high fidelity dna polymerase, by New England Biolabs (1 mentions)
2 protocols using novex wedgewell 14 tris glycine mini protein gels
1
Optimized His-tagged Protein Purification
2
Protein Expression and Purification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All chemicals were used as supplied by vendors without further purification. Imidazole, Invitrogen Novex WedgeWell 14% tris-glycine Mini Protein Gels, NativePAGE 4 to 16% bis-tris Mini Protein Gels, NativeMark Unstained Protein Standard, Isopropy-b-d -thiogalactopyranoside (IPTG), lysozyme, Spectra Multicolor Broad Range Protein Ladder, Pierce BCA protein assay kit, Tris base, Tris HCl, all restriction enzymes, and all cell culture media and reagents were purchased from Fisher Scientific, Inc. (USA). Gibson Assembly Master Mix and Q5 High-Fidelity DNA Polymerase were purchased from NEB (USA). Amicon Ultra-0.5 mL centrifugal units, Benzonase nuclease, and BL21 (DE3) Electrocompetent Cells used for E. coli expression were purchased from MilliporeSigma (USA). Ni-NTA agarose from Gold Biotechnology, Inc. (USA) was used for His-tagged protein purification.
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