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Mirax midi

Manufactured by 3DHISTECH
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Sourced in Hungary

The Mirax Midi is a slide scanner designed for high-quality digitization of histopathology slides. It features a high-resolution camera and automated slide handling capabilities to capture detailed digital images of tissue samples.

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Lab products found in correlation

Caseviewer 2, by 3DHISTECH (2 mentions) Pannoramic viewer 1, by 3DHISTECH (2 mentions) Panoramic viewer 1, by 3DHISTECH (1 mentions) Bond max immunohistochemical staining machine, by Leica (1 mentions) Axiostar plus microscope, by Zeiss (1 mentions) Clone fjk 16s, by Thermo Fisher Scientific (1 mentions) Panoramic viewer, by 3DHISTECH (1 mentions)

5 protocols using mirax midi

1

Quantification of Cell Populations in DG and HC

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Slides were scanned by a digital slide scanner (Mirax Midi, 3DHistech Ltd., Budapest, Hungary), equipped with a Panoramic Viewer 1.15.4, a CaseViewer 2.1 program and a QuantCenter, HistoQuant module (3DHistech Ltd., Budapest, Hungary). For quantifications, all sections derived from each animal were analyzed. In DG and HC, the regions of interest (ROI) were manually outlined. Antibody-positive cell types were counted and quantified from ROIs. The number of stem cells (BrdU+) and neuroblasts (DCX+) were assessed by the observers. The densities (%) of neurons (NeuN+), microglia (Iba1+), and astrocytes (GFAP+) were calculated by the quantification software. To assess cell densities, we divided the total number of counted cells per animal with the DG/HC area, and represented them as cells/mm2 (BrdU+, DCX+) or % (NeuN+, Iba+, GFAP+).
Borbély E., Varga V., Szögi T., Schuster I., Bozsó Z., Penke B, & Fülöp L. (2022). Impact of Two Neuronal Sigma-1 Receptor Modulators, PRE084 and DMT, on Neurogenesis and Neuroinflammation in an Aβ1–42-Injected, Wild-Type Mouse Model of AD. International Journal of Molecular Sciences, 23(5), 2514.
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2

Quantitative Analysis of Neurological Markers

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Slides were scanned by a digital slide scanner (Mirax Midi, 3DHistech Ltd., Budapest, Hungary), equipped with a Pannoramic Viewer 1.15.4, a CaseViewer 2.1 program, and a QuantCenter, HistoQuant module (3DHistech Ltd., Budapest, Hungary). For quantifications, all sections derived from each animal were analyzed (12 slices per animal). In DG and HC, the regions of interest (ROI) were manually outlined. Antibody-positive cell types for all ROIs were counted and quantified. The number of stem cells (BrdU+) and neuroblasts (DCX+) were assessed at the border between GCL and the hilus. The densities (%) of neurons (NeuN+), microglia (Iba1+), astrocytes (GFAP+) and Aβ plaques were calculated by the quantification software. To assess cell densities, we divided the total number of counted cells per animal with the DG/HC area, and presented the results as cells/mm2 (BrdU+, DCX+) or percentages (NeuN+, Iba1+, GFAP+, Aβ plaques).
Szögi T., Borbély E., Schuster I., Bozsó Z., Sántha M., Tóth M.E., Penke B, & Fülöp L. (2022). Examination of Longitudinal Alterations in Alzheimer’s Disease-Related Neurogenesis in an APP/PS1 Transgenic Mouse Model, and the Effects of P33, a Putative Neuroprotective Agent Thereon. International Journal of Molecular Sciences, 23(18), 10364.
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3

Quantitative Analysis of Neuroinflammation

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Slides were scanned by a digital slide scanner (Mirax Midi, 3DHistech Ltd., Budapest, Hungary), equipped with a Pannoramic Viewer 1.15.4, CaseViewer 2.1 program and a QuantCenter, HistoQuant module (3DHistech Ltd., Budapest, Hungary). For quantification, all of the sections were analyzed. The HC and the CTX were manually outlined as the regions of interest (ROI). Antibody-positive cell types from the ROIs were counted and quantified. The percentile densities of microglia (Iba1+), astrocytes (GFAP+), and Aβ plaques were quantified by the quantification software. The cell density was represented as a percentage.
Szögi T., Schuster I., Borbély E., Gyebrovszki A., Bozsó Z., Gera J., Rajkó R., Sántha M., Penke B, & Fülöp L. (2019). Effects of the Pentapeptide P33 on Memory and Synaptic Plasticity in APP/PS1 Transgenic Mice: A Novel Mechanism Presenting the Protein Fe65 as a Target. International Journal of Molecular Sciences, 20(12), 3050.
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4

Immunohistochemical Analysis of CD44 in White Adipose Tissue

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Following terminal anesthesia, vWAT were removed, fixed in 4% formalin, and subsequently embedded in paraffin. Immunohistochemical staining was performed on 4 μm tissue sections using a BOND-MAX Immunohistochemical staining machine (Leica). The sections were incubated with rabbit anti-CD44 antibody (Abcam cat.no: ab157107; 1:1000). A labeling system (Bond Polymer Refine Detection, DS9800, Leica) containing horseradish peroxidase (HRP) conjugated goat anti-rabbit secondary antibody and DAB-3 (3′diaminobenzidine) as the chromogen was used to detect the antigen signal. Cell nuclei were counterstained with hematoxylin. The immunostained sections were digitally scanned using a slide scanner (MiraxMidi, 3DHistech Ltd., Budapest, Hungary). Investigators performing immunohistochemical staining and evaluation were unaware of the group allocation.
Ruppert Z., Neuperger P., Rákóczi B., Gémes N., Dukay B., Hajdu P., Péter M., Balogh G., Tiszlavicz L., Vígh L., Török Z., Puskás L.G., Szebeni G.J, & Tóth M.E. (2024). Characterization of obesity-related diseases and inflammation using single cell immunophenotyping in two different diet-induced obesity models. International journal of obesity (2005).
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5

Immunohistochemical Quantification of Foxp3+ Cells in Heart Allografts

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Heart allografts were fixed in 10% v/v neutral buffered formalin, paraffin-embedded, sectioned, and stained with hematoxylin and eosin. Additionally, sections were assessed by standard immunohistochemical staining for forkhead box P3 (Foxp3; eBioscience, Clone FJK-16s). Whole slide images of stained specimens were generated by scanning with a Mirax MIDI (3D Histech, Budapest, Hungary). Digital images were captured using Panoramic Viewer (Version 1.14; 3D Histech). 10× images were captured using an AxioStar Plus microscope (Zeiss) with a digital camera (AxioCam INC, Zeiss). Foxp3+ cells were quantified by counting positive cells within 2–3 fields per heart.
Rosborough B.R., Raïch-Regué D., Liu Q., Venkataramanan R., Turnquist H.R, & Thomson A.W. (2014). Adenosine triphosphate-competitive mTOR inhibitors: a new class of immunosuppressive agents that inhibit allograft rejection. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 14(9), 2173-2180.
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