Enspire elisa

At a temperature of 25 °C, mix TC (200 nM) with S1–S2 solution and incubate the mixture for 1 h to release S2. Then, add GO/P1 dropwise to the reaction mixture described above. After approximately 30 min of incubation at room temperature the final solution was used for fluorescence detection. For FAM-labeled substrates, use the Multifunctional Microplate Detector (EnSpire ELISA; PerkinElmer, Waltham, MA, USA) scan at 492 nm excitation and 518 nm emission. In the SYBR Green strategy, the fluorescence results were obtained from the multifunctional microplate reader (SLXFA; BioTek, Winooski, VT, USA) using green filters (excitation 485 ± 20, emission 528 ± 20).

IL-1β and tumor necrosis factor-α (TNF-α) were detected with IL-1β (Proteintech Group, INC., China) and TNF-α (KeyGEN BioTECH, China) ELISA kits according to the manufacturer’s recommendations, and the absorbance was measured with an EnSpire ELISA (PerkinElmer, United States).