Au680 automatic analyzer

Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), triglyceride (TG), total cholesterol (TC), high-density lipoprotein-cholesterol (HDL), low-density lipoprotein-cholesterol (LDL), and glucose were measured using an AU680 Automatic Analyzer (Beckman coulter, CA, USA), and lactate dehydrogenase (LDH) was measured using a commercial LDH assay kit (Sigma, St. Louis, MO, USA). Lipid in the liver tissue was extracted using modified methods, as previously described [37 (link)]. Briefly, stored liver tissues were homogenized in chloroform-methanol-distilled water (2 : 1 : 1, v/v/v) using a homogenizer for 1 min, and then centrifuged at 13,000 rpm for 15 min at 4°C. The solvent (chloroform) phase obtained from the extraction was dried and dissolved in methanol, and then the hepatic TG levels were measured using a triglyceride assay kit (Cayman Chemical, Ann Arbor, MI, USA).

Serum samples were analyzed for triglyceride, total cholesterol, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol concentrations using an Olympus AU680 automatic analyzer (Beckman Coulter, Brea, CA, USA), enzymatic reagents (DiaSys, Holzheim, Germany) and secondary standards (Roche Diagnostics, Mannheim, Germany). For glycated hemoglobin (HbA1c), whole blood was analyzed by high pressure liquid chromatography (HPLC) using D-10 (Bio-Rad, Hercules, CA, USA). NT-proBNP was determined using a chemiluminescent microparticle immunoassay (Architect, Abott, IL, United States).