Cryptotanshinone (CPT, purity>98%, molecular weight 296.36) was obtained from National Institutes for Food and Drug Control (Beijing, China). 2.964 mg CPT was dissolved in 1 ml dimethyl sulfoxide (DMSO, Sigma, USA) to make a 10 mM stock solution which was then added to the medium at the indicated concentrations. The methyl thiazolyl tetrazolium (MTT) was obtained from Keygen (Nanjing, China). RPMI-1640 medium and 0.05% trypsin were from Gibco (USA) and Fetal bovine serum was from Corning Cell Gro (Australia). Lipofectamine® 2000 was from Invitrogen (Grand Island, NY, USA). GPER specific agonist G-1 and antagonist G-15 were from Cayman Chemical (Michigan, USA). The following antibodies were used: GPER, PI3K(p85), cyclin A, cyclin B, cyclin D, CDK2 (Abcam, USA), phospho-Akt (p-AKT, Ser473, Cell signaling, Boston, MA, USA). GPER siRNA, non-target siRNA and PI3K inhibitor LY294002 were obtained from Santa Cruz Biotechnology (Texas USA). Fluorescein-Conjugated Goat anti-Rabbit IgG(H + L) was from ZSGB-BIO (Beijing, China) and DAPI was from Solarbio (Beijing, China).
Fluorescein conjugated goat anti rabbit igg h l
Manufactured by ZSGB-BIO
Sourced in China
Fluorescein-Conjugated Goat anti-Rabbit IgG(H + L) is a secondary antibody conjugated with the fluorescent dye fluorescein. It is designed to detect and bind to rabbit primary antibodies directed against the heavy and light chains of immunoglobulin G (IgG).
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Phospho akt p akt ser473, by Cell Signaling Technology (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Ly294002, by Santa Cruz Biotechnology (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Keygen Biotech (1 mentions)
Cyclinb, by Abcam (1 mentions)
Gper specific agonist g 1 and antagonist g 15, by Cayman Chemical (1 mentions)
Gper sirna, by Santa Cruz Biotechnology (1 mentions)
Non target sirna, by Santa Cruz Biotechnology (1 mentions)
Cyclin d, by Abcam (1 mentions)
Hrp goat anti mouse igg, by Immunoway (1 mentions)
2 protocols using fluorescein conjugated goat anti rabbit igg h l
1
Cryptotanshinone Signaling Pathway Study
2
Antibodies for Nephrin, Calpain, and Caspase-3 Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Mouse monoclonal anti-nephrin antibody (Cat#:sc-377246, LOT: G0213) was purchased from Santa Cruz Biotechnology, Inc. (CA, USA). Rabbit polyclonal anti-Calpain 10 antibodies (Cat#: ab28226, LOT: GR342802-7) were purchased from Abcam (Cambridge, UK). Rabbit polyclonal anti-caspase-3 antibodies (Cat#: 9662s, LOT: 18) were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). GAPDH mouse monoclonal antibodies (Cat#:60004-1-lg, LOT:10003343) were purchased from Prointech Group (Rosemont, USA). HRP goat anti-rabbit IgG (Cat#:RS0002, LOT: B0201) and HRP goat anti-mouse IgG (Cat#:RS0001, LOT: B0101) were purchased from ImmunoWay Biotechnology (TX, USA). Rhodamine (TRITC)-Conjugated Goat anti-Mouse IgG(H+L) (Cat#: ZF-0313, LOT:135850) and Fluorescein-Conjugated Goat anti-Rabbit IgG(H+L) (Cat#: ZF-0311, LOT:136851) were purchased from ZSGB-BIO (Beijing, China).
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