CHCl3, MeOH, isopropanol, dimethylformamide (DMF), Rhodamine B octadecyl ester, doxorubicin, paclitaxel, and MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) were purchased from Sigma-Aldrich. Phosphate-buffered saline (PBS) was purchased from Lonza, Belgium.
MKN45P human gastric cancer cells were isolated from parental MKN45 cells and authenticated as described (30 ). MKN45P-luc cells were made by infecting MKN-45P cells with lenti-luciferase (Biogenova, Potomac, MD). SKOV-3 and CT-26 cell lines were purchased from ATCC, UK (SKOV-3 ATCC HBT-77, CT.26 ATCC CLR-2638). Cell lines were used without further authentication. The cells were cultivated in DMEM medium (Lonza, Belgium) with 100 I.U/mL of penicillin, streptomycin, and 10% of heat-inactivated fetal bovine serum (FBS, GE Healthcare, UK).
Athymic nude mice were purchased from Harlan, USA, and Balb/c mice were purchased from Charles River, USA. Animal experimentation procedures were approved by Estonian Ministry of Agriculture, Committee of Animal Experimentation, project #42.
MKN45P human gastric cancer cells were isolated from parental MKN45 cells and authenticated as described (30 ). MKN45P-luc cells were made by infecting MKN-45P cells with lenti-luciferase (Biogenova, Potomac, MD). SKOV-3 and CT-26 cell lines were purchased from ATCC, UK (SKOV-3 ATCC HBT-77, CT.26 ATCC CLR-2638). Cell lines were used without further authentication. The cells were cultivated in DMEM medium (Lonza, Belgium) with 100 I.U/mL of penicillin, streptomycin, and 10% of heat-inactivated fetal bovine serum (FBS, GE Healthcare, UK).
Athymic nude mice were purchased from Harlan, USA, and Balb/c mice were purchased from Charles River, USA. Animal experimentation procedures were approved by Estonian Ministry of Agriculture, Committee of Animal Experimentation, project #42.