Qiacube ht extractor

Manufactured by Qiagen

Sourced in Germany

The QIAcube HT is an automated sample preparation system designed for high-throughput nucleic acid extraction. It is capable of processing multiple samples simultaneously, providing a consistent and efficient way to isolate DNA, RNA, or proteins from a variety of sample types.

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Lab products found in correlation

Qiaamp 96 dna qiacube ht kit, by Qiagen (2 mentions) Nanodrop 2000, by Thermo Fisher Scientific (1 mentions) Nextseq 500 550 high output kit, by Illumina (1 mentions) Covidseq test, by Illumina (1 mentions)

Spelling variants of this product

QIAcube (844 citations) QIAcube HT (130 citations) QiaCube extractor (10 citations)

2 protocols using qiacube ht extractor

Automated DNA Extraction from Throat Samples

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Total DNA was extracted from each swab (tonsil and base tongue) with an automated procedure using a QIAcube HT extractor (Qiagen GmbH, Hilden, Germany) and the dedicated QIAamp 96 DNA QIAcube HT Kit (Qiagen GmbH, Hilden, Germany).
The samples were initially digested in a lysis buffer containing 20 mg/mL of proteinase K and then purified on silica membranes capable of simultaneously binding both genomic and viral DNA. The DNA was washed and then eluted in 150 µL of TE (10 mM Tris-HCl, 0.5 mM EDTA, pH 9.0).
Total DNA concentration and quality were measured using a NanoDrop 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA).

Palmieri A., Lauritano D., Pellati A., Scapoli L., Arcuri C., Baggi L., Gatto R, & Carinci F. (2022). Prevalence of Human Papillomavirus in the Oropharynx of Healthy Individuals in an Italian Population. Journal of Clinical Medicine, 11(7), 1935.

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Wastewater Sequencing for SARS-CoV-2 Detection

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In order to test if the pipeline would perform reliably under industry conditions we also used it with so-called production data from the amedes analytical company. The sequencing was performed as follows:
45 mL of raw wastewater was centrifuged for 10 min with 10,000 x g at 4 °C. Subsequently, the supernatant was prefiltered using Filtropur S 0.45 μm filter units (Sarstedt, Darmstadt, Germany), further transferred to 100 kDa cutoff Amicon Ultra-15 units (PLHK Ultracel-PL Membran, 100 kDa Centrifugation units; Merck Sigma Aldrich Chemie GmbH, Taufkirchen, Germany) and processed according to the manufacturer's manual.
Automated RNA isolation was accomplished using a Qia-Cube HT Extractor using the QIAamp 96 DNA QIAcube HT Kit according to the manufacturer's protocol (Qiagen, Hildesheim, Germany).
Library preparation for NGS sequencing was performed following the complete Illumina SARS-CoV-2 sequencing workflow (Illumina COVIDSeq Test, Illumina, San Diego, USA) including RNA-to-cDNA conversion and SARS-CoV-2 targeted PCR using the ARTIC V3 primer set. The generated libraries were analyzed using NextSeq 550 and 550Dx sequencers with NextSeq 500/550 High Output Kits (v2.5; Illumina #20024906) generating 2 × 37 bp paired-end output.

Schumann V.F., de Castro Cuadrat R.R., Wyler E., Wurmus R., Deter A., Quedenau C., Dohmen J., Faxel M., Borodina T., Blume A., Freimuth J., Meixner M., Grau J.H., Liere K., Hackenbeck T., Zietzschmann F., Gnirss R., Böckelmann U., Uyar B., Franke V., Barke N., Altmüller J., Rajewsky N., Landthaler M, & Akalin A. (2022). SARS-CoV-2 infection dynamics revealed by wastewater sequencing analysis and deconvolution. The Science of the Total Environment, 853, 158931.

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