Serum concentrations of the cytokines interleukin (IL)-1β, IL-2, IL-4, IL-6, IL-10, IL-17A and interferon (IFN)-γ were quantified using the Bio-Plex Pro Human Screening Panel 5plx EXP (Bio-Rad, Hercules, CA, USA) according to the manufacturer’s instructions. For assay analysis, a Luminex® 200 device and Luminex XPonent® software version 3.1 (Luminex, Austin, TX, USA) were used. Tumor necrosis factor (TNF)-α was quantified using ELISA MAX™ Deluxe Sets (BioLegend) according to the recommended protocols of the manufacturer and the Tecan reader Infinite PRO 200 and the i-control™ software (both Tecan Group AG, Männedorf, Switzerland).
Bio plex pro human screening panel 5plx exp
Manufactured by Bio-Rad
Sourced in United States
The Bio-Plex Pro Human Screening Panel 5plx EXP is a multiplex assay designed for the simultaneous detection and quantification of up to 5 human analytes. It provides a versatile platform for screening and profiling of biological samples.
Automatically generated - may contain errors
3 protocols using bio plex pro human screening panel 5plx exp
1
Cytokine Quantification in Serum
2
Multiplex Cytokine Quantification
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The cytokines interleukin (IL)-2, IL-4, IL-10 and interferon (IFN)-γ were quantified in the serum samples using the Bio-Plex Pro Human Screening Panel 5plx EXP (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s instructions. For multiplex assay analysis, a Luminex® 200 device and Luminex XPonent® software version 3.1 (Luminex, Austin, TX, USA) were used. The cytokines tumor necrosis factor (TNF)-α, IL-6 and IL-1β were quantified using ELISA MAX™ Deluxe Sets (BioLegend) according to the recommended protocols of the manufacturer and the Tecan reader Infinite PRO 200 and the i-control™ software (both Tecan Group AG, Männedorf, Switzerland).
3
Quantifying Cytokines and TNF-α in Serum
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The cytokines IL-2, IL-10, and IFN-γ were quantified in the serum samples using the Bio-Plex Pro Human Screening Panel 5plx EXP (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s instructions. For assay analysis, a Luminex® 200 device and Luminex XPonent® software version 3.1 (Luminex, Austin, TX, USA) were used. TNF-α in serum samples was quantified using the ELISA Max Deluxe Set Human TNF-α (BioLegend).
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