Pvpva64

FLQY2 was synthesized by our laboratory (purity  > 98%) [32 ]. Soluplus^®, Poloxamer 407, PVP Va64, and HS 15 were purchased from BASF Co., Ltd (Shanghai, China). PEG4000, PEG6000, and Irinotecan hydrochloride were obtained from Aladdin Bio-chem Technology Co., Ltd (Shanghai, China). Paclitaxel Injection (Albumin-Bound) was purchased from Kelun Pharmaceutical Co., Ltd (Yueyang, China). Human colon cancer cells (HCT 116) and human pancreatic cancer cells (MIA PaCa-2) were acquired from the National Collection of Authenticated Cell Cultures (Shanghai, China). McCoy’s 5A medium and DMEM medium were obtained from SIGMA (Shanghai, China). FBS was purchased from Gibco (Shanghai, China). Penicillin–streptomycin solution (100 ×) was obtained from Solarbio Co., Ltd (Beijing, China). Acetonitrile and ultrapure water for HPLC-grade were purchased from Merck (Darmstadt, Germany). HPLC-grade formic acid and ammonium acetate were purchased from CNW (Shanghai, China). All the other chemicals were of analytical grade and commercially available.

Trans-resveratrol was provided by Ningbo Liwah Pharmaceutical Co., Ltd. (Ningbo, China). Eudragit E PO (powder form of a cationic copolymer based on dimethylaminoethyl methacrylate, butyl methacrylate, and methyl methacrylate with a ratio of 2:1:1), polyvinylpyrrolidone K30 (PVP K30), and polyvinylpyrrolidone vinyl acetate 64 (PVP VA 64) were kindly donated by BASF (Ludwigshafen, Germany). Hydroxylpropylmethyl cellulose (HPMC 6 cp) and hydroxylpropyl cellulose (HPC-L) were kindly donated by Shin-Etsu Chemical Co., Ltd. (Tokyo, Japan), and Nippon Soda Co., Ltd. (Tokyo, Japan), respectively. Eudragit E/HCl (neutralized Eudragit E with hydrochloric acid) powder was prepared as previously reported [29 (link)]. All organic solvents of high-performance liquid chromatography (HPLC) grade were purchased from J.T. Baker (Phillipsburg, NJ, USA). All reagents used were of analytical grade and purchased from Sigma-Aldrich Co., Ltd. (St Louis, MO, USA) or Daejung Chemicals and Metals Co., Ltd. (Siheung-si, Korea).

Itraconazole, naproxen and phenytoin were obtained from Chemie Brunschwig (Basel, Switzerland). Cinnarizine, ketoconazole, probenecid, sodium chloride, sodium hydroxide and sodium phosphate dihydrate were obtained from Sigma-Aldrich (Steinheim, Germany). Dimethyl sulfoxide was obtained from VWR Chemicals (Søborg, Denmark). SIF powder was obtained from Biorelevant (South Croydon, UK). HPMCAS-L was gifted by Dow Wolf Cellulosics (Bomlitz, Germany) and PVPVA64 was obtained from BASF (Basel, Switzerland). All materials were used as received.

PXCM and PHY were purchased from TCI Chemicals (Tokyo, Japan) and Spectrum Chemical (New Brunswick, NJ, USA), respectively. RTV was provided by Abbott Laboratories (now AbbVie Inc., North Chicago, IL, USA). PVP-VA64 (T_g = 108 °C) was purchased from BASF (Ludwigshafen, Germany). All model compounds are poorly soluble drugs as per the biopharmaceutics classification system (BCS) [50 (link)], as detailed in Table 2. HPLC-grade methanol (MeOH) and acetonitrile (ACN) were purchased from Honeywell (Charlotte, NC, USA). Tri-fluoro acetic acid (TFA) and KH₂PO₄ were purchased from Fisher (Hampton, NH, USA). All materials were used as received. All water (H₂O) used was MilliQ grade with 18.2 MΩ resistance.
Physical mixtures were prepared by weighing an appropriate amount of each component into a scintillation vial and mixed with acoustic mixing at 50 G for 2 min using a LabRAM II (Resodyn Corporation, Butte, MT, USA).