Takara premix taq polymerase

Manufactured by Takara Bio

TaKaRa premix Taq polymerase is a ready-to-use solution containing Taq DNA polymerase, buffer, dNTPs, and MgCl2. It is designed for use in a variety of PCR applications.

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Lab products found in correlation

Fast advanced master mix, by Thermo Fisher Scientific (2 mentions) Taqman gene expression cells to ct kit, by Thermo Fisher Scientific (2 mentions) Quantstudio 3 real time pcr machine, by Thermo Fisher Scientific (2 mentions) Rt buffer, by Promega (2 mentions) Biomark hd system, by Standard BioTools (2 mentions) Gene expression master mix, by Thermo Fisher Scientific (1 mentions) Moloney murine leukemia virus, by Promega (1 mentions) Moloney murine leukemia virus m mlv, by Promega (1 mentions)

Spelling variants of this product

Taq polymerase (304 citations) Premix Taq (218 citations) TaKaRa Taq (38 citations) Takara Taq polymerase (8 citations) Premix Taq polymerase (7 citations)

4 protocols using takara premix taq polymerase

Cell-to-CT Taqman Gene Expression Profiling

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A lysis reaction followed by reverse transcription was performed using the kit Taqman Gene Expression Cells-to-CT Kit (ThermoFisher) with ‘Lysis Solution’ followed by the ‘Stop Solution’ at room temperature, and then a reverse transcription with the ‘RT Buffer’, ‘RT Enzyme Mix’, and lysed RNA at 37°C for an hour. Following reverse transcription, cDNA was pre-amplified by adding 2 µL of cDNA from each sample to 8 µL of preamp master mix [5 µL TaKaRa premix Taq polymerase (Clontech), 2.5 µL 0.2X Taqman pooled probe, 0.5 µL H₂O] and thermocycled at 95°C for 3 min, 55°C for 2 min, 72°C for 2 min, then 95°C for 15 s, 60°C for 2 min, 72°C for 2 min for 16–20 cycles, and then a final 10°C hold. Amplified cDNA was then diluted 2:100 in RNase-free H₂O. Each qPCR assay contained the following reagents: 0.5 uL 20X Taqman probe, 2.5 µL RNase-free H₂O, 5 µL Gene Expression Master Mix or Fast Advanced Master Mix (ThermoFisher), and 2 L diluted pre-amplified cDNA. qPCR reactions were performed in triplicate for each Taqman assay of interest on a QuantStudio 3 Real Time PCR Machine (ThermoFisher).

Cleary C.M., Moreira T.S., Takakura A.C., Nelson M.T., Longden T.A, & Mulkey D.K. (2020). Vascular control of the CO2/H+-dependent drive to breathe. eLife, 9, e59499.

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Single-cell mRNA Profiling with Fluidigm

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Plates of FACS-sorted cells were thawed and a combined lysis/denaturation step was performed by incubation at 70°C/4 min, 4°C/5 min, 2.5 µl reverse transcription master mix was then added to each well [1 µl 5× RT buffer (Promega), 0.6 µl 35 mM MgCl₂, 0.25 µl Moloney murine leukemia virus (Promega), 0.1 µl 25 mM dNTPs, 0.5 µl H₂O] and incubated at 37°C/2 min, 42°C/1 min, 50°C/1 s for 40 cycles, then 85°C/5 min, and 4°C hold. Following RT, cDNA was preamplified by adding 2 µl of cDNA from the RT plate to 8 µl of preamp master mix [5 µl TaKaRa premix Taq polymerase (Clontech), 2.5 µl 0.25X TaqMan pool, 0.5 µl H₂O] and thermocycled at 95°C/3 min, 55°C/2 min, 72°C/2 min, then 95°C/15 s, 60°C/2 min, 72°C/2 min for 16 cycles, and 4°C hold. Amplified cDNA was then diluted 1:50 in nuclease-free H₂O and this material was used for qPCR against a curated panel of 48 TaqMan Gene Expression Assays (Table 1) on 48.48 dynamic arrays using a Biomark HD system (Fluidigm).

Mickelsen L.E., Kolling FW I.V., Chimileski B.R., Fujita A., Norris C., Chen K., Nelson C.E, & Jackson A.C. (2017). Neurochemical Heterogeneity Among Lateral Hypothalamic Hypocretin/Orexin and Melanin-Concentrating Hormone Neurons Identified Through Single-Cell Gene Expression Analysis. eNeuro, 4(5), ENEURO.0013-17.2017.

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Single-Cell Gene Expression Profiling

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Plates of FACS-sorted cells were thawed, and a combined lysis and
denaturation step was performed by incubation at 70 °C for 4 min, 4
°C for 5 min. Reverse transcription (RT) master mix (2.5 μl) was
then added to each well (1 μl 5× RT Buffer (Promega), 0.6
μl 35 mM MgCl₂, 0.25 μl Moloney Murine Leukemia Virus
(MMLV) (Promega), 0.1 μl 25 mM dNTPs, 0.5 μl H₂O) and
incubated at 37 °C for 2 min, 42 °C for 1 min and 50 °C for
1 s for 40 cycles, then 85 °C for 5 min, and 4 °C hold. Following
RT, cDNA was pre-amplified by adding 2 μl of cDNA from the RT plate to 8
μl of preamp master mix (5 μl TaKaRa premix Taq polymerase
(Clontech), 2.5 μl 0.25× Taqman pool, 0.5 μl
H₂O) and thermocycled at 95 °C for 3 min, 55 °C for 2
min, 72 °C for 2 min, then 95 °C for 15 s, 60 °C for 2 min,
72 °C for 2 min for 16 cycles, and 4 °C hold. Amplified cDNA was
then diluted 1:50 in nuclease-free H₂O and this material was used for
qPCR against a curated panel of 30 TaqMan Gene Expression Assays (Supplementary Table 2) on
48.48 dynamic arrays using a Biomark HD system (Fluidigm).

Mickelsen L.E., Bolisetty M., Chimileski B.R., Fujita A., Beltrami E.J., Costanzo J.T., Naparstek J.R., Robson P, & Jackson A.C. (2019). Single-cell transcriptomic analysis of the lateral hypothalamic area reveals molecularly distinct populations of inhibitory and excitatory neurons. Nature neuroscience, 22(4), 642-656.

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Gene Expression Analysis via Reverse Transcription and qPCR

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A lysis reaction followed by reverse transcription was performed using the kit Taqman Gene Expression Cells-to-CT Kit (ThermoFisher) with “Lysis Solution” followed by the “Stop Solution” at room temperature, and then a reverse transcription with the “RT Buffer”, “RT Enzyme Mix”, and lysed RNA at 37 °C for an hour. Following reverse transcription, cDNA was pre-amplified by adding 2 µL of cDNA from each sample to 8 µL of preamp master mix [5 µL TaKaRa premix Taq polymerase (Clontech), 2.5 µL 0.2× Taqman pooled probe, 0.5 µL H₂O] and thermocycled at 95 °C for 3 min, 55 °C for 2 min, 72 °C for 2 min, then 95 °C for 15 s, 60 °C for 2 min, 72 °C for 2 min for 16 cycles, and then a final 10 °C hold. Amplified cDNA was then diluted 2:100 in RNase free H₂O. Each qPCR assay contained the following reagents: 0.5 µL 20× Taqman probe, 2.5 µL RNase free H₂O, 5 µL Fast Advanced Master Mix (ThermoFisher), and 2 µL diluted pre-amplified cDNA. qPCR reactions were performed in triplicate for each Taqman assay of interest on a QuantStudio 3 Real Time PCR Machine (ThermoFisher).

Cleary C.M., James S., Maher B.J, & Mulkey D.K. (2021). Disordered breathing in a Pitt-Hopkins syndrome model involves Phox2b-expressing parafacial neurons and aberrant Nav1.8 expression. Nature Communications, 12, 5962.

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