The experiments were performed in perfusion-fixed (4% paraformaldehyde in PBS) MONs or coronal brain slices in a mixture of 4% paraformaldehyde (#P6148, Millipore Sigma, St-Louis, MO) in PBS and 0.025% glutaraldehyde (#O2957, Fisher Scientific, Pittsburgh, PA). Cryoprotection was achieved in 30% sucrose for 16–18 h. Sections of 50 μm (brains) and 16 μm (MONs) thickness were blocked in 20% normal goat/donkey (50% by volume) serum (#G9663 & G6023, Millipore Sigma, St-Louis, MO) and 1% Triton X-100 (#X100, Millipore Sigma, St-Louis, MO) for 60 min at room temperature. Sections were then incubated in primary antibodies prepared in the same solution and kept overnight at 4°C (see Table 1 for antibodies and dilutions used). After several thorough washes in PBS, the tissue was incubated with secondary antibodies, prepared in 2% normal goat serum for 2 h at room temperature. The secondary antibodies used were donkey anti-rabbit Cy2, anti-mouse Cy2 and anti-rabbit Cy3 and anti-mouse Cy3 (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). Sections were double or triple labeled to visualize structures of interest.
O2957
Manufactured by Thermo Fisher Scientific
The O2957 is a laboratory instrument designed to measure the oxygen content in gas samples. It provides accurate and reliable measurements to support various research and analytical applications. The device's core function is to quantify the oxygen levels in the tested gas samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti mouse cy2, by Jackson ImmunoResearch (3 mentions)
P6148, by Merck Group (3 mentions)
G9663, by Merck Group (3 mentions)
G6023, by Merck Group (3 mentions)
Anti rabbit cy3, by Jackson ImmunoResearch (3 mentions)
Anti mouse cy3, by Jackson ImmunoResearch (3 mentions)
Tecnai g20 twin, by Thermo Fisher Scientific (1 mentions)
D5652, by Merck Group (1 mentions)
4 protocols using o2957
1
Immunohistochemistry of Brain Tissue
2
Ultrastructural Detection of Autophagosomes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed in 0.1 M sodium phosphate buffer (pH 7.4, PBS; Sigma, D5652–10 L) containing 4% formaldehyde (Fisher Chemical, F79) plus 1% glutaraldehyde (Fisher Chemical, O2957) for 4 h at 4 °C. After collection, samples were sent to the Wuhan Institute of Virology, Chinese Academy Of Sciences for detection. Autophagosomes are defined as double layer membrane structure containing cytoplasmic contents (mitochondria, lipid, damaged organelles, etc.) waiting to be degraded. Results were observed in a transmission electron microscope (FEI, Tecnai G20 TWIN, USA)
3
Immunohistochemistry of Brain Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The experiments were performed in perfusion-fixed (4% paraformaldehyde in PBS) MONs or coronal brain slices in a mixture of 4% paraformaldehyde (#P6148, Millipore Sigma, St-Louis, MO) in PBS and 0.025% glutaraldehyde (#O2957, Fisher Scientific, Pittsburgh, PA). Cryoprotection was achieved in 30% sucrose for 16–18 h. Sections of 50 μm (brains) and 16 μm (MONs) thickness were blocked in 20% normal goat/donkey (50% by volume) serum (#G9663 & G6023, Millipore Sigma, St-Louis, MO) and 1% Triton X-100 (#X100, Millipore Sigma, St-Louis, MO) for 60 min at room temperature. Sections were then incubated in primary antibodies prepared in the same solution and kept overnight at 4°C (see Table 1 for antibodies and dilutions used). After several thorough washes in PBS, the tissue was incubated with secondary antibodies, prepared in 2% normal goat serum for 2 h at room temperature. The secondary antibodies used were donkey anti-rabbit Cy2, anti-mouse Cy2 and anti-rabbit Cy3 and anti-mouse Cy3 (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). Sections were double or triple labeled to visualize structures of interest.
4
Immunohistochemistry of Brain Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The experiments were performed in perfusion-fixed (4% paraformaldehyde in PBS) MONs or coronal brain slices in a mixture of 4% paraformaldehyde (#P6148, Millipore Sigma, St-Louis, MO) in PBS and 0.025% glutaraldehyde (#O2957, Fisher Scientific, Pittsburgh, PA). Cryoprotection was achieved in 30% sucrose for 16–18 h. Sections of 50 μm (brains) and 16 μm (MONs) thickness were blocked in 20% normal goat/donkey (50% by volume) serum (#G9663 & G6023, Millipore Sigma, St-Louis, MO) and 1% Triton X-100 (#X100, Millipore Sigma, St-Louis, MO) for 60 min at room temperature. Sections were then incubated in primary antibodies prepared in the same solution and kept overnight at 4°C (see Table 1 for antibodies and dilutions used). After several thorough washes in PBS, the tissue was incubated with secondary antibodies, prepared in 2% normal goat serum for 2 h at room temperature. The secondary antibodies used were donkey anti-rabbit Cy2, anti-mouse Cy2 and anti-rabbit Cy3 and anti-mouse Cy3 (Jackson ImmunoResearch Laboratories, Inc., West Grove, PA). Sections were double or triple labeled to visualize structures of interest.
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