Gallic acid (CAS 149-91-7) and chlorogenic acid 95% (CAS 327-97-9) were supplied by Sigma, Life Science, Shanghai, China. Apple pectin (CAS 9000-09-5) was purchased from LABChem, Lisbon, Portugal; maltodextrin (CAS 32671-12) from Fargon, Barcelona, Spain; and gum Arabic from Guinama, Valencia, Spain. Water-soluble starch from potato (CAS 9005-84-9) was supplied from PanReac-Applichem, Darmstadt, Germany. Soybean lecithin (CAS 8002-43-5; purity of 90%) was obtained from Alfa Aesar, Thermofisher, Kandel, Germany. Cholesterol (CAS C8667-25G; purity ≥ 99%) was supplied by Sigma, Life Science, St. Louis, MO, USA. Absolute ethanol (≥99.8% and 96% purity), dichloromethane (≥99.9%), and HPLC grade analytical solvents were purchased from Honeywell (Hessen, Germany). Relite EXA90 (ion exchange resin) DiaionTM HP20LX (spherical porous polystyrene resin) were both kindly supplied by the Resindion group of Mitsubishi Chemical, Binasco, Italy. Sodium phosphate dibasic heptahydrate (Na2HPO4·7H2O) and sodium phosphate monobasic monohydrate (NaH2PO4·H2O) were purchased from PanReac, Barcelona, Spain. Ultra-pure water was obtained from Seralpur PRO 60 CN and Seradest LFM 20 water purification systems.
Sodium phosphate dibasic heptahydrate
Manufactured by ITW Reagents
Sourced in Spain
Sodium phosphate dibasic heptahydrate is a chemical compound with the formula Na₂HPO₄·7H₂O. It is a crystalline solid that is soluble in water and is commonly used as a buffer in biochemical and analytical applications.
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2 protocols using sodium phosphate dibasic heptahydrate
1
Detailed Extraction and Purification Protocol
2
Cloud point determination of triblock copolymers
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All studied triblock copolymers studied in this work were purchased from Sigma-Aldrich, namely Pluronic L-31, L-35, 31R1 and 10R5 (see Table S1). Both the imidazolium: McIlvaine buffer (0.18 M) at pH 7.0 was used as solvent, being composed of sodium phosphate dibasic heptahydrate (purity ≥ 99%) and citric acid monohydrate (purity ≥ 99%), which were obtained from Panreac AppliChem.
The cloud point determination was established in McIlvaine buffer solutions, since this is the medium of preference to work with pH sensitive or labile biomolecules, and at copolymer concentrations ranging from 0.5 to 17.5 wt% and SAIL concentrations of 0.3 wt%. For each system, three replicas were determined, and the average value calculated. The cloud points were measured through the visual determination of the onset turbidity of a solution heated in a temperature controlled water bath with an error of ± 0.01 o C following a methodology described elsewhere. 30, 31 The full results are shown in Figure S1.
The cloud point determination was established in McIlvaine buffer solutions, since this is the medium of preference to work with pH sensitive or labile biomolecules, and at copolymer concentrations ranging from 0.5 to 17.5 wt% and SAIL concentrations of 0.3 wt%. For each system, three replicas were determined, and the average value calculated. The cloud points were measured through the visual determination of the onset turbidity of a solution heated in a temperature controlled water bath with an error of ± 0.01 o C following a methodology described elsewhere. 30, 31 The full results are shown in Figure S1.
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