The major chemical reagents for synthesis were purchased from Alfa Aesar, Sigma Aldrich Co or Aladdin Reagent Database Inc (Shanghai). Ethyl 5,12-dioxo-5,12-dihydroindolizino[2,3-g]quinoline- 6-carboxylate (1 ) was prepared by our lab according to our reported method [9 (link)]. The common solvents were obtained from local commercial suppliers and used without further purification. Plasmid pBR322 DNA and purified calf thymus DNA topoisomerase I was purchased from TakaRa Biotechnology (Dalian) Co., Ltd., unless otherwise mentioned. One unit of Top1 was defined as the amount that relaxes 0.5 μg of pBR322 DNA at 37 °C for 30 min. Chemical reaction courses were monitored by silica gel GF254 thin layer chromatography. Melting points were determined in open capillary tubes on a MPA100 Optimelt Automated Melting Point System without being corrected. Nuclear magnetic resonance spectra were recorded on a Bruker AVANCE III 400 MHz spectrometer using tetramethylsilane as an internal reference. Mass spectra were analyzed on an Agilent 6120 (Quadrupole LC-MS) mass spectrometer. The high-resolution mass spectra were analyzed on an SHIMADZU LCMS-IT-TOF mass spectrometer. All compounds tested for biological activities were analyzed by HPLC and their purities were more than 95%.
6120 quadrupole lc ms mass spectrometer
Manufactured by Agilent Technologies
Sourced in United States
The 6120 Quadrupole LC-MS is a mass spectrometer designed for liquid chromatography-mass spectrometry (LC-MS) applications. It features a quadrupole mass analyzer and is capable of performing full-scan and selected ion monitoring (SIM) analyses. The 6120 Quadrupole LC-MS is used for the detection and quantification of a wide range of molecular compounds in various sample types.
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2 protocols using 6120 quadrupole lc ms mass spectrometer
1
Synthesis and Topoisomerase I Inhibition of Indolizino[2,3-g]quinoline Derivatives
2
Synthesis and Characterization of Organic Compounds
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The chemical reagents and solvents were commercially available from Hi-media Laboratories Private Limited and were used without further purification. The termination of the reaction was confirmed by performing thin layer chromatography on aluminium sheets coated with silica gel G containing fluorescent indicators, F 254. The mobile phase for the development of the plate was ethyl acetate and petroleum ether (1:1). Melting point values were determined on a DRK Digital melting point apparatus and are reported uncorrected. IR spectra were recorded using KBr powder on Shimadzu diffuse reflectance attachment (DRS-800) mounted on a Shimadzu IR-Affinity spectrometer. 1 H NMR spectra were obtained on a Bruker Avance 300 MHz instrument in DMSO-d 6 (Tetramethylsilane as the internal standard). Chemical shift values are reported in parts per million (d, ppm). Mass spectra were run using a atmospheric pressureelectron spray ionization 6120 Quadrupole LC/MS mass spectrometer (Agilent Technologies, California, USA).
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