Calcium chloride (Ca2+) and Calmodulin (CaM), >95% pure, were purchased from Sigma Chemical (St. Louis, MO). 2',5'-ADP-Sepharose 4B was the product of Amersham Pharmacia Biotech (Piscataway, NJ). L-[14C]arginine was from NEN (Boston, MA). NADPH, tetrahydrobiopterin, NG-nitro-L-arginine methyl ester (L-NAME), diphenyleneiodonium (DPI) and other reagents were purchased from Sigma unless otherwise indicated. Both bovine WT eNOS pcDNA3 and S1179D eNOS pcDNA3 plasmids were purchased from www.addgene.com .
Calmodulin cam
Manufactured by Merck Group
Sourced in China
Calmodulin (CaM) is a small calcium-binding protein found in all eukaryotic cells. It functions as a ubiquitous intracellular calcium receptor and signal transducer, playing a central role in the regulation of a wide range of cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
K2hpo4, by Tianjin Guangfu (3 mentions)
Sodium hydroxide (naoh), by Tianjin Guangfu (3 mentions)
Sodium chloride (nacl), by Tianjin Guangfu (3 mentions)
Potassium chloride (kcl), by Tianjin Guangfu (3 mentions)
Mgcl2, by Tianjin Guangfu (3 mentions)
Cacl2, by Tianjin Guangfu (3 mentions)
Tetrahydrobiopterin, by Merck Group (1 mentions)
Ng nitro l arginine methyl ester l name, by Merck Group (1 mentions)
Diphenyleneiodonium dpi, by Merck Group (1 mentions)
2 5 adp sepharose 4b, by Cytiva (1 mentions)
Nadph, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Mncl2, by Tianjin Guangfu (1 mentions)
Haucl4, by Sinopharm (1 mentions)
2 protocols using calmodulin cam
1
Purification and Activation of eNOS
2
Sensitive Detection of Tetracycline Antibiotics
Check if the same lab product or an alternative is used in the 5 most similar protocols
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All chemicals were of analytical reagent grade and were used as received.
Distilled water (18.2 MΩ cm -1 ) was used throughout the current study. Oxytetracycline (OTC), tetracycline (TC), hydrogen tetrachloroaurate(III) tetrahydrate (HAuCl4• 4H2O), and streptavidin (SA) were purchased from Heowns Biochem Technology Co., Ltd. (Tianjin, China). Doxycycline (DC) was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). Hydroxylammonium chloride (NH2OH• HCl) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). NaOH, K2HPO4, and metal salts including NaCl, KCl, MgCl2, CuCl2, MnCl2, FeCl3, and CaCl2 were purchased from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). Phenylalanine (Phe), histidine (His), arginine (Arg), valine (Val), and isoleucine (Ile) were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA) and calmodulin (CaM) were purchased from Sigma-Aldrich (Beijing, China). Other reagents were obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China).
A stock solution of HAuCl4 (1%, w/v) was prepared by dissolving 1 g HAuCl4 in 100 mL of ultrapure water and stored at 4°C. The preparation of 0.1 M of Mcllvaine-EDTA buffer (pH 4) was carried out by dissolving 5.9 g trisodium citrate dihydrate, 13.8 g Na2HPO4•12H2O, and 18.6 g Na2EDTA•12H2O in 500 mL of ultrapure water.
Distilled water (18.2 MΩ cm -1 ) was used throughout the current study. Oxytetracycline (OTC), tetracycline (TC), hydrogen tetrachloroaurate(III) tetrahydrate (HAuCl4• 4H2O), and streptavidin (SA) were purchased from Heowns Biochem Technology Co., Ltd. (Tianjin, China). Doxycycline (DC) was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). Hydroxylammonium chloride (NH2OH• HCl) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). NaOH, K2HPO4, and metal salts including NaCl, KCl, MgCl2, CuCl2, MnCl2, FeCl3, and CaCl2 were purchased from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). Phenylalanine (Phe), histidine (His), arginine (Arg), valine (Val), and isoleucine (Ile) were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA) and calmodulin (CaM) were purchased from Sigma-Aldrich (Beijing, China). Other reagents were obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China).
A stock solution of HAuCl4 (1%, w/v) was prepared by dissolving 1 g HAuCl4 in 100 mL of ultrapure water and stored at 4°C. The preparation of 0.1 M of Mcllvaine-EDTA buffer (pH 4) was carried out by dissolving 5.9 g trisodium citrate dihydrate, 13.8 g Na2HPO4•12H2O, and 18.6 g Na2EDTA•12H2O in 500 mL of ultrapure water.
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