Anti gata3 bv421

Manufactured by BioLegend

Sourced in United States

Anti-GATA3-BV421 is a fluorochrome-conjugated antibody that binds to the GATA3 transcription factor. GATA3 is a key regulator of Th2 cell differentiation and function. This product can be used for the detection and analysis of GATA3-expressing cells in flow cytometry applications.

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Anti-GATA3 (4 citations)

3 protocols using anti gata3 bv421

Phenotypic Characterization of Dendritic Cells and ILC2s

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DCs were assessed using the antibodies of anti-CD11c-PC5.5; anti-CD80-FITC; anti-HLA-DR-APC-Cy7 and anti-CD86-PE-Cy7. PBMCs or ILC2s were stained with Fixable Viability Dye-eF506 and the following specific mAbs: Lineage cocktail (CD2, CD3, CD14, CD16, CD19, CD56, CD235a, FceR1)-FITC (all from eBioscience, San Diego, Calif, USA); anti-CRTH2-PE (BD Pharmingen, USA) and anti-CD127-PE-Cy7 (eBioscience, San Diego, Calif, USA). For intracellular cytokine assay, PBMCs or ILC2s were stimulated with phorbol myristate acetate (PMA), ionomycin, and brefeldin A (BFA) for 5 h, and then, the cells were fixed, permeabilized, and stained with anti-IL-13-BV421 (BioLegend, San Diego, CA, USA). For GATA3 levels detection, the cells were processed with the FoxP3/Transcription Factor Staining Buffer Set before staining with anti-GATA3-BV421(BioLegend, San Diego, CA, USA). After staining, samples were analyzed on a CytoFLEX Flow Cytometer (Beckman Coulter, Hercules, CA, USA).

Liu X.Q., Peng Y.Q., Huang L.X., Li C.G., Kuang P.P., Chen D.H., Wu Z.C., He B.X., Zhou Z.R, & Fu Q.L. (2023). Dendritic cells mediated by small extracellular vesicles derived from MSCs attenuated the ILC2 activity via PGE2 in patients with allergic rhinitis. Stem Cell Research & Therapy, 14, 180.

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Characterization of Murine ILC2 Cells

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Testes were collected from adult C57BL/6 (JAX®mice, stock no. 000664) mice and enzymatically and mechanically dissociated into a single cell suspension enriched for interstitial cells as described above. The single cell suspension is stained with a series of cell surface markers including anti-CD8-BV570 (1:300; Biolegend 100739), anti-CD3-PE/Cy7 (1:300; Biolegend 100219), anti-CD4-BV510 (1:300; Biolegend 100449), anti-IL7r-PE/Cy5 (1:300; Biolegend 135015). However, for intracellular staining - the ILCII cells were first incubated with PMA (10 ng / mL), ionomycin (10 μM), with the addition of Golgi-stop reagent (BD Bioscience, San Jose, CA) for 4 h at 37°C and subsequently stained for anti-IL4-PE (1:300; Biolegend 504103), anti-IL-13-APC (1:300; Novus 011818), and anti-Gata3-BV421 (1:300; Biolegend 653813) for 30 minutes prior to flow cytometry.

Green C.D., Ma Q., Manske G.L., Shami A.N., Zheng X., Marini S., Moritiz L., Sultan C., Gurczynski S.J., Moore B.B., Tallquist M.D., Li J.Z, & Hammoud S.S. (2018). A comprehensive roadmap of murine spermatogenesis defined by single-cell RNA-seq. Developmental cell, 46(5), 651-667.e10.

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Comprehensive Immune Cell Analysis

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The following antibodies and kits were used for immunofluorescence and flow-cytometry analysis: anti-CD4-PErCP/Cy5.5 (100434, BioLegend), anti-CD4-BV605 (100548, BioLegend), anti-CD4-PE (100408, BioLegend), anti-CD4-BV650 (100469, BioLegend), anti-CD25-Alexa647 (102020, BioLegend), anti-CD25-PE/Cy7 (102016, BioLegend), anti-CD25-FITC (101908, BioLegend), anti-CD69 BV510 (104532, BioLegend), anti-CD8a-PE (100708, BioLegend), anti-RORγt-Vio515 (130-124-078, MiltenyiBiotec), anti-GATA3-BV421 (653814, BioLegend), anti-GATA3-PE (653804, BioLegend) anti-TBET- PerCP/Cy5.5 (644806, BioLegend), anti-Tbet-BV421 (644816, BioLegend) anti-FOXP3-APC (130-093-013, MiltenyiBiotec), anti-CD11b- PerCP/Cy5.5 (101228, BioLegend), anti-LY6G-BV421 (127628, BioLegend), anti-LY6C-BV605 (128036, BioLegend), anti- TCRβ -BV421 (109229, BioLegend, anti-TCRγδ-PE (118108, BioLegend), anti CD3 (100202; BioLegend), anti-CD68 (ABIN181836, antibodies-online), anti GFP (ab6673, abcam), anti-IgA (NB7501, Novus), anti-E-cadherin (610182, BD Transduction Laboratories), anti-Ki-67 (IHC-00375, Bethyl), anti-F4/80-PE (123110, BioLegend) anti-FITC IgG (SAB4600050, sigma), anti-rat-Cy5 (112-175-143, Jackson ImmunoResearch), anti-goat-FITC (205-095-108, Jackson ImmunoResearch).

Glaubitz J., Wilden A., Frost F., Ameling S., Homuth G., Mazloum H., Rühlemann M.C., Bang C., Aghdassi A.A., Budde C., Pickartz T., Franke A., Bröker B.M., Voelker U., Mayerle J., Lerch M.M., Weiss F.U, & Sendler M. (2023). Activated regulatory T-cells promote duodenal bacterial translocation into necrotic areas in severe acute pancreatitis. Gut, 72(7), 1355-1369.

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