Log In Sign up
The largest database of trusted experimental protocols

Apc conjugated donkey anti rabbit igg

Manufactured by Jackson ImmunoResearch
Visit Supplier
Sourced in United Kingdom

APC)-conjugated donkey anti-rabbit IgG is a secondary antibody used for detection and visualization purposes. It is composed of donkey-derived antibodies that specifically bind to rabbit immunoglobulin G (IgG), and are conjugated with the fluorescent dye APC (Allophycocyanin).

Automatically generated - may contain errors

Lab products found in correlation

Ab10135, by Abcam (1 mentions) Ab18433, by Abcam (1 mentions) Ab117529, by Abcam (1 mentions) Bsa bovine serum albumin, by Merck Group (1 mentions) Alexa 488 conjugated donkey anti goat igg, by Jackson ImmunoResearch (1 mentions) Fixable viability dye efluor 780, by Thermo Fisher Scientific (1 mentions) Liberase tm research grade, by Roche (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Vectashield antifade mounting medium, by Vector Laboratories (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Cd80 pe, by Thermo Fisher Scientific (1 mentions) Cd86 pe, by Thermo Fisher Scientific (1 mentions) Cd4 rm4 5 pe cy7, by BD (1 mentions) Mtor mab 7c10, by Cell Signaling Technology (1 mentions) Gfp d5.1 mab, by Cell Signaling Technology (1 mentions) B220 fitc, by BD (1 mentions) Cxcr3 apc, by Thermo Fisher Scientific (1 mentions) Pd l2 pe, by Thermo Fisher Scientific (1 mentions) Cd11b pe cy7, by BD (1 mentions)

3 protocols using apc conjugated donkey anti rabbit igg

1

Immunofluorescence Analysis of ETB Receptor

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-SM22 (1:100, ab10135, Abcam, UK), Goat isotope control IgG (5 μg/mL, ab18433 Abcam), Rabbit anti-ETB receptor (1:100, ab117529, Abcam), Rabbit isotope control IgG (10 μg/mL, ab18433, Abcam), Allophycocyanin (APC)-conjugated donkey anti-rabbit IgG (1:100, Jackson ImmunoResearch), Alexa 488-conjugated donkey anti-goat IgG (1:100, Jackson ImmunoResearch), Fixable Viability Dye eFluor 780 (eBioscience), Liberase TM Research Grade (Roche), German coverslip with Poly-L-lysine coating on both sides cat no H-12-pII Neuvitro, USA, Vectashield Antifade Mounting Medium (Vector Laboratories, USA), BSA (Bovine Serum Albumin, Sigma Aldrich), FBS (Fetal Bovine Serum, Sigma Aldrich), Triton X-100 (Sigma Aldrich).
Radziwon-Balicka A., Degn M., Johansson S.E., Warfvinge K, & Edvinsson L. (2017). A novel multicolor flow-cytometry application for quantitative detection of receptors on vascular smooth muscle cells. PLoS ONE, 12(10), e0186504.
+ Open protocol
+ Expand
2

ZIKV Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were induced with doxycycline for 24 hours and then were stained
with sera or primary antibodies against ZIKV for 1 hour at 4°C and
subsequently stained with Allophycocyanin (APC)-conjugated goat anti-mouse IgG
antibody (Jackson ImmunoResearch Laboratory: #115-136-072), APC-conjugated goat
anti-human IgG (H+L) antibody (Jackson ImmunoResearch Laboratory: #109-136-098),
or APC-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratory:
#711-136152) for 30 min. Flow cytometry was performed to determine the cellular
expression of ZIKV proteins. Images were acquired on an LSR II flow cytometer
(BD Biosciences) and analyzed with FlowJo software (Tree Star).
Chen X., Anderson L.J., Rostad C.A., Ding L., Lai L., Mulligan M., Rouphael N., Natrajan M.S., McCracken C, & Anderson E.J. (2020). Development and Optimization of a Zika Virus Antibody-Dependent Cell-Mediated Cytotoxicity (ADCC) Assay. Journal of immunological methods, 488, 112900.
+ Open protocol
+ Expand
3

Comprehensive Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: mTOR mAb (7C10), phospho-S6 (Ser235/236) (D57.2.2E)-APC, phospho-p70 S6 kinase (Thr389) (108D2) mAb, phosphor-AKT (Thr308) (C31E5E) mAb, phospho-Akt (Ser473) (D9E) mAb, GFP (D5.1) mAb, SHP-2 (D50F2) mAb (Cell Signaling Technology); APC-conjugated Donkey-anti-Rabbit IgG (Jackson ImmunoResearch); CD3e (145-2C11)-FITC, CD4 (RM4-5)-PE-Cy7, Vβ4 (KT4)-PE, CD11c-APC, CD11b-PE-Cy7, B220-FITC, CD19-PE-Cy7 and PD-L1-PE (BD Biosciences); F4/80-PE and PD-1-PE-Cy7 (BioLegend); CD71-APC and CD98-PE, CD90.1 (Thy-1.1)-PE, CXCR3-APC, CD80-PE, CD86-PE and PD-L2-PE (eBioscience); PD-1 (J43) Ab (Novus); T-bet-FITC and SH-PTP2 (C-18) (Santa Cruz Biotechnology).
Chen W., Wan X., Ukah T.K., Miller M.M., Barik S., Cattin-Roy A.N, & Zaghouani H. (2016). Antigen-Specific Immune Modulation Targets mTORC1 Function to Drive Chemokine Receptor-Mediated T Cell Tolerance. Journal of immunology (Baltimore, Md. : 1950), 197(9), 3554-3565.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!