Rat igg2a 2a3 violetfluor450 isotype

Manufactured by Cytek Biosciences

The Rat IgG2a (2A3) violetFluor450 Isotype is a fluorochrome-conjugated antibody used in flow cytometry applications. It serves as an isotype control for rat IgG2a antibodies.

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Lab products found in correlation

Cd73 efluor450, by Thermo Fisher Scientific (2 mentions) Cd4 rm4 5 percpcy5, by BD (2 mentions) Cd8 53 6.7 bv650, by BD (2 mentions) Cd11c n418 pe, by BD (2 mentions) Foxp3 fjk16s pe, by BD (2 mentions) Fr4 pe cy7, by Thermo Fisher Scientific (2 mentions) Pdl1 percp efluor710, by Thermo Fisher Scientific (2 mentions) Mhc 2 1 ab efluor450, by Thermo Fisher Scientific (2 mentions) Igm fab apc, by Jackson ImmunoResearch (2 mentions) Rat igg1 hrpn percp cy5.5 isotype, by Cytek Biosciences (2 mentions) Lsr 2 fortessa cytometer, by BD (2 mentions) Cd3 pe, by BD (1 mentions) Nk1.1 pk136, by BD (1 mentions) Cd19 bv605, by BD (1 mentions)

2 protocols using rat igg2a 2a3 violetfluor450 isotype

Multi-color Flow Cytometry Panel for Immune Profiling

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Antibodies for flow cytometry and histology include CD3 PE, CD4 (RM4-5) PerCPCy5.5, CD8 (53-6.7) BV650, CD11c (N418) PE, FOXP3 (FJK16S) PE, were purchased from BD Biosciences (San Jose, CA); NK1.1 (PK136), CD11b (M1/70), CD11c (N418), B220 (RA3-6B2), and F4/80 in APC-eFluor780; PD1 (J43) FITC, CD73 eFluor450, FR4 PE-Cy7, PDL1 PerCP-eFluor710, MHC-II I-Ab eFluor450, IL7R PE, and all ELISpot antibodies were purchased from eBiosciences (San Diego, CA), and IgM (Fab′) APC was purchased from Jackson Immunoresearch (West Grove, PA). Rat IgG1 (HRPN) PerCP-Cy5.5 Isotype and Rat IgG2a (2A3) violetFluor450 Isotype were purchased from Tonbo Biosciences (San Diego, CA). Cells from enriched fractions were analyzed on an LSR-II Fortessa cytometer (BD Biosciences, San Jose CA) and data was analyzed in FlowJo (Treestar, Ashland OR). Intracellular cytokine staining was done using BD Cytofix/Cytoperm reagents and protocols (BD Biosciences, San Jose CA). Anti-IL10 (JES5-16E3, PE, eBiosciences), TGFB1(LAP) (TW7-16B4, ef710, eBiosciences), IFNγ (XMG1.2, BV421, BD Horizon), and TNFα (MP6-XT22, AF488, eBiosciences) were stained by this procedure.

Manlove L.S., Berquam-Vrieze K.E., Pauken K.E., Williams R.T., Jenkins M.K, & Farrar M.A. (2015). Adaptive immunity to leukemia is inhibited by cross-reactive induced regulatory T cells. Journal of immunology (Baltimore, Md. : 1950), 195(8), 4028-4037.

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Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies for flow cytometry include CD3 PE, CD4 (RM4-5) PerCPCy5.5, CD8 (53-6.7) BV650, CD11c (N418) PE, FOXP3 (FJK16S) PE, CD80 (16-10A1) APC, CD86 (GL1) PE-Cy7, CD19 BV605, B220 (RA3-6B2) Horizon V500, IFNgamma (XMG1.2) BV650, LAP (TW7-16B4) PE, TNF alpha (MP6-XT22) BV421, IL17A (TC11-18H10) AlexaFluor488, and PSGL1 (2PH1) BV421 purchased from BD Biosciences (San Jose, CA); NK1.1 (PK136), CD11b (M1/70), CD11c (N418), B220 (RA3-6B2), and F4/80 in APC-eFluor780; PD1 (J43) FITC, CD73 eFluor450, FR4 PE-Cy7, PDL1 PerCP-eFluor710, MHC-II I-Ab eFluor450, IL10 (JESS-16E3) PE, Granzyme B (NGZB) PE-Cy7, GARP (YGIC86) eFluor450, and all ELISpot antibodies were purchased from eBiosciences (San Diego, CA), and IgM (Fab′) APC was purchased from Jackson Immunoresearch (West Grove, PA). Rat IgG1 (HRPN) PerCP-Cy5.5 Isotype and Rat IgG2a (2A3) violetFluor450 Isotype were purchased from Tonbo Biosciences (San Diego, CA). Cells from enriched fractions were analyzed on an LSR-II Fortessa cytometer (BD Biosciences, San Jose CA) and data was analyzed in FlowJo (Treestar, Ashland OR).

Manlove L.S., Schenkel J.M., Manlove K.R., Pauken K.E., Williams R.T., Vezys V, & Farrar M.A. (2016). Heterologous vaccination and checkpoint blockade synergize to induce anti-leukemia immunity. Journal of immunology (Baltimore, Md. : 1950), 196(11), 4793-4804.

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