Triplicate samples at 0.5, 3, 9, 12, and 24 h from the four different experimental groups (ABPPk, NGF, serum deprivation, and normal SCs) were collected. According to the manufacturer’s protocol, the 60 ribonucleic acid (RNA) samples were extracted using the mirVana miRNA Isolation Kit (Ambion). RNA integrity was determined using the Agilent 2100 Bioanalyzer (Agilent Technologies, USA). Strand-specific RNA-seq libraries were constructed, and the samples were sequenced on the Illumina Hiseq X Ten sequencing platform (OEbiotech, Shanghai, China) with 150 bp paired-end runs. All subsequent analyses were performed using clean reads. The sequencing data were uploaded to the sequence read archive (SRA) database (accession number: SRP253388, PRJNA613365).
Hiseq x ten
Manufactured by OE Biotech
Sourced in China
The Hiseq X Ten is a high-throughput DNA sequencing system designed for large-scale genomic studies. It utilizes Illumina's proprietary sequencing-by-synthesis technology to generate high-quality, massively parallel DNA sequencing data. The system is capable of producing up to 1.8 terabases of data per run, making it suitable for a wide range of genomic applications, including whole-genome sequencing, exome sequencing, and targeted gene panels.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions)
Mirvana mirna isolation kit, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Truseq small rna sample prep kit, by Illumina (1 mentions)
2 protocols using hiseq x ten
1
Temporal RNA-seq of neural stem cells
2
Small RNA Sequencing from Gonads
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total RNA was extracted using TRizol® (Invitrogen, Carlsbad, CA) reagent from the gonads. Subsequently, small RNA was isolated from total RNA to prepare libraries via the TruSeq Small RNA Sample Prep Kits (Illumina, Unites States). Small RNA was ligated 5′ and 3′ adapter and then reverse transcribed to construct complementary (c)DNA using SuperScript II Reverse Transcriptase (Invitrogen, Unites States). Afterwards, the obtained cDNA libraries were purified by RNA Gel Electrophoresis and validated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Unites States). Finally, the RNA-seq libraries were sequenced on the Illumina HiSeq X Ten (OE Biotech, Shanghai, China).
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