For a subset of 37 participants, all of whom were CP subjects, we analyzed the salivary cytokines IL-1β, IL-6, IL-8, IL-10, and TNF-α. The analysis of cytokines in saliva was performed using a CBA Cytokine Inflammatory Kit (Becton Dickinson, CA, USA) for the detection of TNF-α, IL-1β, IL-6, IL-8, IL-10. All analyses were performed in duplicate. Briefly, 25μL of fluorescent particles conjugated to antibodies specific for each cytokine were added to 25 μL of the saliva and incubated for 1 h at room temperature away from light. Subsequently, 25 μL of the secondary antibody conjugated to a fluorochrome was added to the mixture and incubated for 2 h at room temperature. The results were compared to a standard curve with serially diluted cytokines. The particles were washed to remove unbound antibodies, resuspended in the wash buffer, and analyzed using a BD Accuri (BD Biosciences). Data acquisition was performed using BD-Accuri C6 Software, and concentrations were determined using FCAP software v.3.0 (BD Biosciences).
Cba cytokine inflammatory kit
Manufactured by BD
Sourced in United States
The BD CBA Cytokine Inflammatory Kit is a multiplex assay designed to quantitatively measure multiple cytokines in a single sample. The kit utilizes flow cytometry technology to detect and analyze the concentration of specific cytokines in biological samples.
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Lab products found in correlation
2 protocols using cba cytokine inflammatory kit
1
Salivary Cytokine Analysis in Cerebral Palsy
2
Cytokine Analysis in Saliva using CBA
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The analysis of cytokines in saliva was performed using a CBA Cytokine Inflammatory Kit (Becton Dickinson, USA) to detect IL-1β, IL-6, IL-8, IL-10, IL-12p70 and TNF-α. All analyses were performed in duplicate.
In brief, 25 μl of fluorescent particles conjugated to antibodies specific to each cytokine were added to 25 μl of the saliva and incubated for one hour at room temperature, away from light. Then, 25 μl of the secondary antibody conjugated to a fluorochrome was added to the mixture and incubated for two hours at room temperature. The results were compared to a standard curve with serially diluted cytokines. The particles were washed to remove the unbound antibodies, resuspended in wash buffer and analyzed using a BD Accuri (BD Biosciences). Data acquisition was performed using BD Accuri C6 Software, and concentrations were determined using FCAP Software v.3.0 (BD Biosciences).
In brief, 25 μl of fluorescent particles conjugated to antibodies specific to each cytokine were added to 25 μl of the saliva and incubated for one hour at room temperature, away from light. Then, 25 μl of the secondary antibody conjugated to a fluorochrome was added to the mixture and incubated for two hours at room temperature. The results were compared to a standard curve with serially diluted cytokines. The particles were washed to remove the unbound antibodies, resuspended in wash buffer and analyzed using a BD Accuri (BD Biosciences). Data acquisition was performed using BD Accuri C6 Software, and concentrations were determined using FCAP Software v.3.0 (BD Biosciences).
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