Tissue and cellular malondialdehyde (MDA), glutathione (GSH) content, and non-heme iron were measured with commercially available Malondialdehyde (MDA) Test Kit (A003-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China), Micro Reduced Glutathione (GSH) Assay Kit (A006-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) and Iron Assay Kit (A039-2-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s instructions.
Micro reduced glutathione gsh assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The Micro Reduced Glutathione (GSH) Assay Kit is a laboratory tool designed to quantify the levels of reduced glutathione in biological samples. It provides a reliable and efficient method for measuring this important antioxidant molecule.
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3 protocols using micro reduced glutathione gsh assay kit
1
Oxidative Stress Biomarker Quantification
2
Assaying Cellular Redox Status
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CAL27 and SCC25 cells were treated with PBS or oxaliplatin, and then they were collected and homogenized in ice-cold PBS. After being centrifuged for 15 minutes at 12,000 rpm at 4° C, the supernatants were collected and used for the analysis. Total GSH and SOD detection assays were performed using the Micro Reduced Glutathione (GSH) Assay Kit (Nanjing Jiancheng, Nanjing, China) and Total SOD Detection Kit (Nanjing Jiancheng, Nanjing, China) according to the manufacturer's protocol.
3
Heart Tissue Iron and GSH Assay
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The method described above was used to homogenize heart tissues, and centrifugation was used to obtain the supernatant. The iron assay kit (Applygen, Beijing, China) and micro reduced glutathione GSH Assay Kit (Jiancheng, Nanjing, China) were used to measure the levels of iron and GSH. The absorbance of iron and the GSH assay kit were read at wavelengths of 550 and 405 nm, respectively. The concentrations of both were calculated using a standard curve.
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