Cisplatin was manufactured by Fresenius Kabi (Lake Zurich, IL) and obtained from the pharmacy at the University of Pittsburgh Cancer Institute. Cetuximab was from Brystol-Myers Squibb (New York, NY), and panitumumab was from Amgen (Thousand Oaks, CA). Water-soluble gefitinib and SB203580 were obtained from Tocris Bioscience. Anti-human antibodies used for flow cytometry staining included Alexa 488-p-S727-STAT1er727, PE-pSTAT1Tyr701, Alexa 647-STAT1 N terminus, Pacific Blue-p-p38 and corresponding isotype controls, all from BD Biosciences. Anisomycin, antibodies to p-S727-STAT1 and p-Y701-STAT1 for immunohistochemistry of tumor specimens, and antibodies for Western blots (total STAT1 primary and anti-rabbit secondary) were obtained from Cell Signaling Technology. Annexin V stain and Annexin buffer were obtained from BD Biosciences. Zombie Aqua viability dye was obtained from BioLegend and used at a dilution of 1/500. Lipopolysaccharide and β-actin antibody were obtained from Sigma. The APO-BrdU TUNEL assay kit for flow cytometry was from Life Technologies.
Annexin 5 stain
Manufactured by BD
Sourced in United States
Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine (PS). Annexin V stain is used to detect and quantify apoptotic cells in flow cytometry and fluorescence microscopy applications.
Automatically generated - may contain errors
Lab products found in correlation
Cisplatin, by Fresenius (1 mentions)
Annexin buffer, by BD (1 mentions)
Zombie aqua viability dye, by BioLegend (1 mentions)
Anisomycin, by Cell Signaling Technology (1 mentions)
Sb203580, by Bio-Techne (1 mentions)
Antibodies for western blot, by Cell Signaling Technology (1 mentions)
β actin antibody, by Merck Group (1 mentions)
Lipopolysaccharide, by Merck Group (1 mentions)
Panitumumab, by Amgen (1 mentions)
2 protocols using annexin 5 stain
1
Evaluation of Cisplatin, Cetuximab, and Panitumumab Efficacy
2
Apoptosis Assessment via Annexin V
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Apoptotic activity due to neuronal induction was assessed using Annexin V stain (Catalog # 556547, BD Pharmingen, USA). Neurospheres from each group were gently dissociated into single-cell format, resuspended in 1X binding buffer, and then incubated with FITC-conjugated Annexin V and propidium iodide for 15 minutes, according to the manufacturer’s recommended protocol. Apoptotic activity was observed under a confocal microscope, and quantitative results were obtained by flow cytometry analysis.
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