Antibodies against phospho akt473

LPS (Escherichia coli 0111:B4) was from Sigma-Aldrich. Dextran sulfate sodium (DSS, MW 47,000; MP Biomedicals) was purchased from MP Biomedicals. Antibodies against phospho-AKT473, phospho-ERK1/2, phosphor-STAT1, AKT, ERK1/2, STAT1, GAPDH were purchased from Cell Signaling Technology, PHLPP was from EMD Millipore, β-ACTIN was from Santa Cruz. FITC conjugated anti-mouse LY6G antibody, Alexa Fluor 647 conjugated anti-mouse CD182 (CXCR2) Antibody, APC/Cy7 and PE conjugated anti-mouse/human CD11B antibody were from Biolegend. ELISA Kit for TNFα, IL-1β, and IL-6 were from eBioscience, respectively. MPO ELISA Kit from R&D system. leukotriene B4 (LTB4), N-Formyl-Met-Leu-Phe (fMLF), and percoll were purchased from Sigma-Aldrich. EasySep™ Mouse Neutrophil Enrichment Kit was purchased from Stemcell Technologies. MIP-1α, fibronectin, and G-CSF was from Peprotech. Anti-PMP70 was from ThermoFisher Scientific. Anti-LAMP was from Abcam.

LPS (Escherichia coli 0111:B4), TUDCA were obtained from Sigma Aldrich. Anti-FPR2 (M-73) antibody was obtained from Santa Cruz Biotechnology. Antibodies against phospho-AKT⁴⁷³, LAMP1, phospho-CREB, phospho-MSK1, GAPDH were purchased from Cell Signaling Technology. FITC conjugated anti-mouse Ly6G antibody, PE conjugated anti-mouse CD195(CCR5) antibody, PE/Cy7 conjugated anti-mouse Ly6C antibody, APC/Cy7 and PE conjugated anti-mouse/human CD11b antibody were from Biolegend (San Diego, CA). FPRL1 (bs-3654R) antibody was from Bioss Antibodies. Anti-citrullinated Histone H4 antibodywas fromMillipore (Billerica,MA). ELISA Kit for TNF-α, IL1-β, IL-6 and IL-10 from eBioscience respectively. MPO ELISA Kit from R&D system.