τaq pcr master mix 1

The extracellular HBV titers were measured by quantitative real-time PCR (qPCR) as described previously [31 (link)]. Briefly, HBV genomic DNA was purified from the culture supernatant using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). For conventional PCR analysis of HBV DNA, genomic DNA was amplified using 2× Τaq PCR Master mix 1 (BioFACT, Daejeon, Korea) and a primer pair, HBV 1399F (5′-TGG TAC CTG CGC GGG ACG TCC TT-3′) and HBV 1632R (5′-AGC TAG CGT TCA CGG TGG TCT CC-3′), as described previously [24 (link)]. Additionally, qPCR for HBV was performed as previously described [31 (link)]. Briefly, HBV DNA was amplified using SYBR premix Ex Taq II (Takara Bio, Kusatsu, Japan) and a primer pair, HBV 379F (5′-GTG TCT GCG TTT TAT CA-3′) and HBV 476R (5′-GAC AAA CGG GCA ACA TAC CTT-3′), using a Rotor Gene qPCR machine (Qiagen).

The HBV titer in the culture supernatant was measured using qPCR as previously described [31 (link)]. Briefly, HBV genomic DNA was purified from the culture supernatant using the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany, Cat No. 51306). For conventional PCR analysis, the purified HBV genomic DNA was amplified using 2 × Τaq PCR Master Mix 1 (BioFACT, Daejeon, Republic of Korea, Cat No. ST301-19h) and a primer pair, HBV 1399F (5′-TGGTACCTGCGCGGGACGTCCTT-3′) and HBV 1632R (5′-AGCTAGCGTTCACGGTGGTCTCC-3′). For qPCR analysis, HBV DNA was amplified with SYBR Premix Ex Taq II (Takara Bio, Kusatsu, Japan, Cat No. RR82LR), HBV 379F (5′-GTGTCTGCGGCGTTTTATCA-3′), and HBV 476R (5′-GACAAACGGGCAACATACCTT-3′) using a Rotor Gene qPCR instrument (Qiagen).