The Luminex 100 Bio-Plex cytokine assay (Bio-Rad Laboratories, Inc; Hercules CA, USA) was used to determine plasma levels of: IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL12(P40), IL-12(P70), IL-17, Eotaxin, Keratinocyte Chemoattractant (KC), Monocyte Chemoattractant Protein-1 (MCP-1), Monocyte Inflammatory Protein-1α (MIP-1α) and Tumor Necrosis Factor-α (TNF-α). Statistical analysis was performed for the cytokines that reached the limit of detection (IL-1β, IL-12, eotaxin, MCP1 and IL–1α).
Luminex 100 bio plex
Manufactured by Bio-Rad
Sourced in United States
The Luminex 100 Bio-Plex is a multiplex assay system that utilizes fluorescent-encoded beads to detect and quantify multiple analytes simultaneously in a single sample. The system is designed to perform high-throughput, sensitive, and accurate analysis of proteins, nucleic acids, and other biomolecules.
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Lab products found in correlation
2 protocols using luminex 100 bio plex
1
Luminex Assay for Plasma Cytokines
2
Cytokine Analysis of Basolateral Media
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Post-exposure basolateral media was removed following each time point and stored at À80 C until cytokine analysis.
Cytokines were evaluated by Luminex V R xMAP V R technology (cat. # HCYTOMAG-60K MILLIPLEX MAP, EMD Millipore, Burlington, MA) as per manufacturer's protocols using a Bio-Rad Luminex 100 Bio-Plex. The cytokines evaluated were interferon gamma-induced protein 10 (IP-10), interleukin 8 (IL-8), and interleukin 1 alpha (IL-1a). A mixture of 10 ng/mL tumor necrosis factor-alpha (TNF-a) and 10 ng/mL of interleukin 1 beta (IL-1b) was added to the basolateral media and exposed to the tissues for the same duration as the CRPs. Before analysis, samples were thawed at room temperature and subsequently maintained at room temperature during processing. All samples were centrifuged before pipetting onto the assay plate. Each sample was analyzed in duplicate.
Cytokines were evaluated by Luminex V R xMAP V R technology (cat. # HCYTOMAG-60K MILLIPLEX MAP, EMD Millipore, Burlington, MA) as per manufacturer's protocols using a Bio-Rad Luminex 100 Bio-Plex. The cytokines evaluated were interferon gamma-induced protein 10 (IP-10), interleukin 8 (IL-8), and interleukin 1 alpha (IL-1a). A mixture of 10 ng/mL tumor necrosis factor-alpha (TNF-a) and 10 ng/mL of interleukin 1 beta (IL-1b) was added to the basolateral media and exposed to the tissues for the same duration as the CRPs. Before analysis, samples were thawed at room temperature and subsequently maintained at room temperature during processing. All samples were centrifuged before pipetting onto the assay plate. Each sample was analyzed in duplicate.
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