Single cell suspensions of freshly dissected thymuses were obtained and either mechanically suspended or enzymatically digested as previously described5 (link), 74 (link) and counted using the Z2 Coulter Particle and Size Analyzer (Beckman Coulter, USA). For studies sorting rare populations of cells in the thymus, multiple identically treated thymuses were pooled so that sufficient number of cells could be isolated; however, in this instance separate pools of cells were established to maintain individual samples as biological replicates.
Z2 coulter particle and size analyzer
Manufactured by Beckman Coulter
Sourced in United States
The Z2 Coulter Particle and Size Analyzer is a laboratory instrument used for the measurement and analysis of particle size and count. It utilizes the Coulter principle to detect and characterize particles suspended in an electrolyte solution. The instrument provides accurate and reproducible data on particle size distribution and concentration.
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Lab products found in correlation
3 protocols using z2 coulter particle and size analyzer
1
Thymus Single Cell Isolation
2
Thymus Cell Isolation and Quantification
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Single cell suspensions of freshly dissected thymuses were obtained and either mechanically suspended or enzymatically digested using 0.15% Collagenase D (Sigma, 11088882001) and 0.1% DNase 1 (Sigma, 10104159001) in DMEM, as previously described (Dudakov et al., 2012 (link); Velardi et al., 2014 (link)), and counted using the Z2 Coulter Particle and Size Analyzer (Beckman Coulter, USA). For studies sorting rare populations of cells in the thymus, multiple identically-treated thymuses were pooled so that sufficient number of cells could be isolated; however, in this instance separate pools of cells were established to maintain individual samples as biological replicates.
3
Thymus Cell Isolation and Quantification
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