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Tabletop sem tm3030plus

Manufactured by Hitachi
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The Tabletop SEM TM3030Plus is a compact scanning electron microscope (SEM) designed for desktop use. It provides high-resolution imaging capabilities for various materials and samples. The core function of the Tabletop SEM TM3030Plus is to capture detailed images of specimens by using a focused beam of electrons to scan the surface.

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Lab products found in correlation

Ffc 1100, by JEOL (1 mentions) Sputter coater, by Cressington (1 mentions)

Close spelling variants of this product

TM3030Plus (109 citations) TM3030Plus SEM (11 citations)

5 protocols using tabletop sem tm3030plus

1

Scanning Electron Microscopy of Biofilm Formation

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The specimen surface was observed before and after biofilm formation using scanning electron microscopy (SEM). A set of specimens (n = 3/group) not used for microbiological procedures was mounted on stubs with conductive tape, sputter coated (JEOL FFC-1100, Japan), and observed with SEM (Tabletop SEM TM3030 Plus, Hitachi, Schaumburg, IL, USA) at 15 KV acceleration voltage. Four randomly selected fields at two different magnifications (500X and 5000X) were recorded for each specimen.
Specimens undergoing SEM analysis after biofilm formation (n = 3/group) were placed into a cacodylate-buffered 2% glutaraldehyde fixative solution (pH = 7.4) for 48 h. The specimens were then passed through a graded ethanol series (50, 70, 80, 85, 90, 95, and 100%, v/v). Finally, they were subjected to critical point drying (Critical-Point Dryer, EMS 850, Hatfield, PA, USA), mounted on stubs with conductive tape, sputter coated, and observed with SEM at 15 KV acceleration voltage. Four randomly selected fields at two different magnifications (500X and 5000X) were recorded for each specimen.
Ionescu A.C., Brambilla E., Azzola F., Ottobelli M., Pellegrini G, & Francetti L.A. (2018). Laser microtextured titanium implant surfaces reduce in vitro and in situ oral biofilm formation. PLoS ONE, 13(9), e0202262.
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2

Ultrastructural Analysis of Ae. aegypti Midguts

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Ae. aegypti mosquitoes, 7 days post emergence, were offered a non-infectious bloodmeal, sorted and housed as described in the main text. Ten midguts from unfed control and engorged mosquitoes were dissected at 24 and 72 hpbm and fixed in a 2% paraformaldehyde/ 2.5% glutaraldehyde solution containing 0.1% (w/v) CaCl2 and 1% (w/v) sucrose buffered in 100mM Na cacodylate (pH 7.3). Samples were fixed at 4°C for three days and postfixed in 1% (w/v) OsO4 in the same buffer at room temperature for one hour. Fixed specimens were dehydrated through a graded ethanol and acetone series and imaged on a Hitachi Tabletop SEM TM3030Plus.
Armstrong P.M., Ehrlich H.Y., Magalhaes T., Miller M.R., Conway P.J., Bransfield A., Misencik M.J., Gloria-Soria A., Warren J.L., Andreadis T.G., Shepard J.J., Foy B.D., Pitzer V.E, & Brackney D.E. (2019). Successive Bloodmeals Enhance Virus Dissemination within Mosquitoes and Increase Transmission Potential. Nature microbiology, 5(2), 239-247.
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3

Ultrastructural Analysis of Ae. aegypti Midguts

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Ae. aegypti mosquitoes, 7 days post emergence, were offered a non-infectious bloodmeal, sorted and housed as described in the main text. Ten midguts from unfed control and engorged mosquitoes were dissected at 24 and 72 hpbm and fixed in a 2% paraformaldehyde/ 2.5% glutaraldehyde solution containing 0.1% (w/v) CaCl2 and 1% (w/v) sucrose buffered in 100mM Na cacodylate (pH 7.3). Samples were fixed at 4°C for three days and postfixed in 1% (w/v) OsO4 in the same buffer at room temperature for one hour. Fixed specimens were dehydrated through a graded ethanol and acetone series and imaged on a Hitachi Tabletop SEM TM3030Plus.
Armstrong P.M., Ehrlich H.Y., Magalhaes T., Miller M.R., Conway P.J., Bransfield A., Misencik M.J., Gloria-Soria A., Warren J.L., Andreadis T.G., Shepard J.J., Foy B.D., Pitzer V.E, & Brackney D.E. (2019). Successive Bloodmeals Enhance Virus Dissemination within Mosquitoes and Increase Transmission Potential. Nature microbiology, 5(2), 239-247.
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4

Imaging Mites in Low-Vacuum SEM

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Mites were directly placed on aluminum studs on a disk-sample holder of a Hitachi TM3030Plus Tabletop SEM (Tarrytown, NY) equipped with a proprietary, highly sensitive low-vacuum secondary electron detector. The low-vacuum mode allowed us to image the mites without any coating or dehydration processing.
Cómbita-Heredia O., Gulbronson C.J., Ochoa R., Quintero-Gutiérrez E.J., Bauchan G, & Klompen H. (2021). Size, shape, and direction matters: Matching secondary genital structures in male and female mites using multiple microscopy techniques and 3D modeling. PLoS ONE, 16(8), e0254974.
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5

Scanning Electron Microscopy of Additive Films

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Scanning electron microscopy (SEM) was utilized to investigate the morphology of the films after the addition of RBP and RBE. Micrographs of the surface of the films with the highest percentages of these additives were obtained using a Hitachi TM3030 Plus Tabletop SEM. Samples were mounted on an aluminum sample holder and were coated with iridium for 30 s using a sputter coater (Cressington, UK) prior to obtaining the images at magnifications of 5×, 200× and 500× under high vacuum and an accelerating voltage of 10 kV.
Cejudo‐Bastante M.J., Cejudo‐Bastante C., Cran M.J., Heredia F.J., & Bigger S.W. (2020). Optical, structural, mechanical and thermal characterization of antioxidant ethylene vinyl alcohol copolymer films containing betalain-rich beetroot. Food Packaging and Shelf Life, 24, 100502-100502.
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