Xanthohumol

Soluble recombinant human TRAIL (rhsTRAIL) was obtained from PeproTech Inc. (Rocky Hill, NJ, USA). Xanthohumol was purchased from Alexis Biochemicals (San Diego, CA, USA) and dissolved in DMSO (50 mM) to a final concentration of 0.1% (v/v) in the culture media. The purity of tested Xanthohumol was ≥98% (HPLC).

Xanthohumol ((2E)-1-[2,4-dihydroxy-6-methoxy-3-(3-methyl-2-buten-1-yl)phenyl]-3-(4-hydroxyphenyl)-2-propen-1-one) was purchased from Alexis Biochemicals (San Diego, CA, USA). Epigallocatechin-3-gallate (EGCG) was purchased by Sigma Aldrich (Sigma-Aldrich, St Louis, MO, USA). STO-609 was purchased from Sigma-Aldrich (St Louis, MO, USA) and Compound C from CalbioChem (Darmstadt, Germany). The ON-TARGET plus SMART pool siRNAS targeting AMPK α1 (PRKAA1 L-0502700) and ON-TARGET plus Nontargeting Pool negative control siRNA (D-001810) were purchased from Dharmacon (Lafayette, CO). Lipofectamine RNAiMAX (catalog number 13778-075) was purchased from Invitrogen (Eugene, OR).