Nasopharyngeal swabs were collected in viral transport media (MSwab; Copan, Brescia, Italy) and transferred to the virology diagnostic laboratory for further real-time multiplex PCR analysis. For detection of respiratory pathogens, RNA was extracted from respiratory specimens using the QIAamp viral RNA minikit (Qiagen, Hilden, Germany). Amplification and detection of viral RNA was performed with FTD respiratory pathogens-21 PCR (Fast-Track Diagnostics; Abbott, Wiesbaden, Germany) on a LightCycler 480 instrument II (Roche, Mannheim, Germany) detecting the following pathogens: influenza virus A, influenza virus A/H1N1, influenza virus B, RV, RSV, BV, AV, PIV types 1–4, CoVs (NL63, 229E, OC43, and HKU1), PV, EV other than RV, HMPV, and Mycoplasma pneumoniae. For further data analysis, influenza A, influenza A/H1N1, and influenza B were clustered as influenza virus; CoVs NL63, 229E, OC43, and HKU1 were clustered as CoV; and PIV 1–4 were clustered as PIV. RV and EV infection can be distinguished by this assay. However, positive signals for RV and EV indicate that the sample is EV positive according to the manufacturer. As a result, conclusions about RV and EV coinfection are limited and were therefore excluded from the analysis.
Mswab
Manufactured by Copan
Sourced in Italy
MSwab is a laboratory sample collection device designed for the safe and efficient collection of specimens. It features a sterile swab and a transport medium vial to preserve the sample during transportation to the laboratory for analysis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mswab
1
Multiplex PCR Respiratory Pathogen Detection
2
Pediatric Respiratory Infection Evaluation
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Between November 2016 and March 2017, we prospectively collected 533 nasopharyngeal swabs (NPS) in the outpatient department of the Center of Childhood and Adolescent Medicine Heidelberg, Germany. Study inclusion criteria were I) age < 18 years, II) clinical symptoms of an acute respiratory infection, and III) indication for hospitalization according to the clinical judgment of the attending physician. Patients with clinical symptoms of an acute respiratory infection included cases of upper respiratory tract infection (URTI), otitis media, croup, bronchiolitis, bronchitis and pneumonia.
Nasopharyngeal swabs were collected by local staff in 1 ml viral transport media (VTM; MSwab; Copan, Brescia, Italy). 200 μl of the VTM were directly used for point-of-care testing with the Alere i RSV assay. The remaining sample was transferred to the virology diagnostic laboratory, and stored in 200 μl aliquots at −80 °C until further analysis.
Attending pediatricians prospectively reported medical information on a standardized data sheet, including the duration of clinical symptoms, demographic and clinical data.
Nasopharyngeal swabs were collected by local staff in 1 ml viral transport media (VTM; MSwab; Copan, Brescia, Italy). 200 μl of the VTM were directly used for point-of-care testing with the Alere i RSV assay. The remaining sample was transferred to the virology diagnostic laboratory, and stored in 200 μl aliquots at −80 °C until further analysis.
Attending pediatricians prospectively reported medical information on a standardized data sheet, including the duration of clinical symptoms, demographic and clinical data.
3
Pediatric Respiratory Infections Surveillance
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During the 2015-2016 winter season (1 November 2015 to 1 April 2016), a nasopharyngeal swab (NPS) was obtained from children hospitalized with symptoms of acute respiratory tract infection at the Center for Childhood and Adolescent Medicine in Heidelberg, Germany.
NPS were collected in 1 ml universal transport medium (article number 806E013N; MSwab; Copan, Brescia, Italy) at the time of admission to inpatient care. After collection, all NPS specimens were refrigerated at 4°C. Within 24 h, aliquots were prepared and stored at −80°C for further analyses.
Demographic and clinical information were collected prospectively. Physicians were asked to specify a diagnosis on admission (i.e., reason for hospitalization) based on their clinical judgment. Diagnoses were classified to upper respiratory tract infection (URTI; including cases of rhinitis, pharyngitis, otitis media, and croup), lower respiratory tract infection (LRTI; including cases of bronchitis, bronchiolitis, and pneumonia), or other admission diagnoses (including, among others, cases of febrile convulsions, fever of unknown origin, and gastroenteritis).
NPS were collected in 1 ml universal transport medium (article number 806E013N; MSwab; Copan, Brescia, Italy) at the time of admission to inpatient care. After collection, all NPS specimens were refrigerated at 4°C. Within 24 h, aliquots were prepared and stored at −80°C for further analyses.
Demographic and clinical information were collected prospectively. Physicians were asked to specify a diagnosis on admission (i.e., reason for hospitalization) based on their clinical judgment. Diagnoses were classified to upper respiratory tract infection (URTI; including cases of rhinitis, pharyngitis, otitis media, and croup), lower respiratory tract infection (LRTI; including cases of bronchitis, bronchiolitis, and pneumonia), or other admission diagnoses (including, among others, cases of febrile convulsions, fever of unknown origin, and gastroenteritis).
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