Frozen sections were fixed with 4% neutral buffered paraformaldehyde (Bmassay, Beijing, China) for 10 min and then blocked with PBST containing 1% BSA for 2 h at room temperature. After blocking, the sections were incubated with primary antibody including NPR1 (1:100, Thermo Fisher Scientific, Waltham, MA, USA), ITGB4 (1:400, Proteintech, Wuhan, Hubei, China), ICAM-1 (1:200, Proteintech, Wuhan, Hubei, China), and CD31 (1:100, R&D, Minneapolis, MN, USA) at 4 °C overnight. Subsequently, the sections were incubated with secondary antibody Cy3-labeled donkey anti-rabbit IgG (1:200, Biolegend. San Diego, CA, USA) or Cy5-affinipure donkey anti-goat IgG (1:200, Jackson ImmunoResearch, West Grove, PA, USA) at room temperature 1 h. The nucleus was stained with Hochest3342 (Beyotime, Shanghai, China). The sections were examined by confocal microscope (Carl Zeiss, Oberkochen, Germany).
Cy5 affinipure donkey anti goat igg
Manufactured by Jackson ImmunoResearch
Sourced in United States
Cy5-affinipure donkey anti-goat IgG is a secondary antibody conjugated with the fluorescent dye Cy5. It is designed to detect and bind to goat immunoglobulin G (IgG) proteins in various immunoassays and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
Confocal microscope, by Zeiss (1 mentions)
Itgb4, by Proteintech (1 mentions)
Cy3 labeled donkey anti rabbit igg, by BioLegend (1 mentions)
Icam 1, by Proteintech (1 mentions)
Vecta shield with dapi, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Vectashield with dapi, by Vector Laboratories (1 mentions)
Alexa fluor 633 phalloidin, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
2 protocols using cy5 affinipure donkey anti goat igg
1
Immunofluorescence Analysis of Vascular Markers
2
Visualizing Drosophila Fat Body Structure
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The fat body from the 2 nd or 3 rd instar larvae were dissected in Grace's Medium and then fixed in 4% formaldehyde in PBS for 15 min before immunofluorescence staining. For membrane staining, fixed fat bodies were washed twice for 5 min in PBS and then were incubated with 0.165 μM Alexa
Fluor 488 phalloidin or Alexa Fluor 633 phalloidin (Invitrogen) in PBSTG for 30 min at RT. Then samples were rinsed in PBST twice for 5min each and mounted in Vecta shield with DAPI (Invitrogen). For cytoophida immunostaining, goat anti CTPS (1:400, Santa Cruz Catalogue no.33304) was used as the primary antibody, and Cy5-AffiniPure donkey anti-goat IgG
(1:1000, Jackson Immuno Research Laboratories, Inc., Catalogue no. 705-175-147) as the secondary antibody. Tissues were mounted in the antifading mounting medium with DAPI (Invitrogen). Then samples were rinsed in PBS twice for 5min each and mounted in Vecta shield with DAPI (Vector Labs).
Fluor 488 phalloidin or Alexa Fluor 633 phalloidin (Invitrogen) in PBSTG for 30 min at RT. Then samples were rinsed in PBST twice for 5min each and mounted in Vecta shield with DAPI (Invitrogen). For cytoophida immunostaining, goat anti CTPS (1:400, Santa Cruz Catalogue no.33304) was used as the primary antibody, and Cy5-AffiniPure donkey anti-goat IgG
(1:1000, Jackson Immuno Research Laboratories, Inc., Catalogue no. 705-175-147) as the secondary antibody. Tissues were mounted in the antifading mounting medium with DAPI (Invitrogen). Then samples were rinsed in PBS twice for 5min each and mounted in Vecta shield with DAPI (Vector Labs).
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