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Immpact novared peroxidase kit

Manufactured by Vector Laboratories
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The ImmPact NovaRED peroxidase kit is a laboratory product designed for use in immunohistochemistry (IHC) and other related applications. The kit provides a chromogenic substrate that produces a red-colored reaction when used with peroxidase-conjugated secondary antibodies. This allows for the visualization and detection of target antigens in biological samples.

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NovaRed (137 citations) ImmPACT NovaRED (26 citations) NovaRed kit (15 citations) ImmPACT NovaRED Peroxidase Substrate Kit (7 citations) ImmPACT NovaRED kit (3 citations) Vector ImmPACT NovaRED peroxidase substrate kit (2 citations)

2 protocols using immpact novared peroxidase kit

1

Histopathology and Viral Antigen Visualization

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For visualization of histopathology, sections of paraffin-embedded formalin-fixed tissue were stained with hematoxylin and eosin on a SAKURA VIP 6 tissue processor at the University of Texas Medical Branch Surgical Pathology Laboratory. For visualization of viral antigen, tissue sections were deparaffinized and stained with a SARS-CoV-2 N-specific rabbit monoclonal antibody (Sino Biological #40143-R001) at a dilution of 1:30,000 followed by an anti-rabbit HRP-linked secondary (Cell Signaling #7074). Signal was developed with ImmPact NovaRED peroxidase kit (Vector Labs # SK-4805).
Schindewolf C., Lokugamage K., Vu M.N., Johnson B.A., Scharton D., Plante J.A., Kalveram B., Crocquet-Valdes P.A., Sotcheff S., Jaworski E., Alvarado R.E., Debbink K., Daugherty M.D., Weaver S.C., Routh A.L., Walker D.H., Plante K.S, & Menachery V.D. (2022). SARS-CoV-2 Uses Nonstructural Protein 16 to Evade Restriction by IFIT1 and IFIT3. bioRxiv.
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2

Histopathological Analysis of SARS-CoV-2 Infection

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For visualization of histopathology, sections of paraffin-embedded formalin-fixed tissue were stained with hematoxylin and eosin on a SAKURA VIP 6 tissue processor at the University of Texas Medical Branch Surgical Pathology Laboratory. For visualization of viral antigen, tissue sections were deparaffinized and stained with a SARS-CoV-2 N-specific rabbit monoclonal antibody (Sino Biological No. 40143-R001) at a dilution of 1:30,000 followed by an anti-rabbit horseradish peroxidase (HRP)-linked secondary (Cell Signaling No. 7074). A signal was developed with an ImmPact NovaRED peroxidase kit (Vector Labs No. SK-4805). Viral antigen staining was scored blinded on a scale of 0 (none) to 3 (most) in 0.25 score increments.
Schindewolf C., Lokugamage K., Vu M.N., Johnson B.A., Scharton D., Plante J.A., Kalveram B., Crocquet-Valdes P.A., Sotcheff S., Jaworski E., Alvarado R.E., Debbink K., Daugherty M.D., Weaver S.C., Routh A.L., Walker D.H., Plante K.S, & Menachery V.D. (2023). SARS-CoV-2 Uses Nonstructural Protein 16 To Evade Restriction by IFIT1 and IFIT3. Journal of Virology, 97(2), e01532-22.
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