Neuroblastoma cell lines SK-N-BE(2)-C (ATCC® CRL-2268™) were used in this study. Cells were cultured in DMEM supplemented with 10% foetal calf serum (FCS, Invitrogen, Australia) and 1% L-glutamine (Life Technologies, USA). Normal lung fibroblasts, MRC-5 (ATCC® CCL-171™), were cultured as described previously 28 . Primary normal human astrocytes (NHA, Lonza, Australia) were cultured as recommended in astrocyte basal medium (ABM) supplemented with astrocyte growth medium (AGM) Single Kit Supplements & Growth Factors (Lonza, Australia). Upon receipt from ATCC, master stocks were prepared and cells for experiments were passaged for less than 6 months. SK-N-BE(2)-C and MRC-5 master stock cells were validated by PCR (STR profiling) and stored in the Children's Cancer Institute Cell Bank. Cell lines were routinely screened and found to be free of mycoplasma contamination. All experiments were performed using the same fetal calf serum to keep constant the amount of copper in the cell culture media at 18ng/mL. Cells incubations were conducted at standard cell culture conditions (37°C, 5% CO2, 95% humidity).
Crl 2268
Manufactured by American Type Culture Collection
The CRL-2268 is a cell line from the American Type Culture Collection (ATCC). It is derived from human embryonic kidney cells. The core function of this cell line is to provide a source of cells for various research and experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
L glutamine, by Thermo Fisher Scientific (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Ccl 171, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Corning (1 mentions)
Penicillin, by Corning (1 mentions)
Sk n as, by American Type Culture Collection (1 mentions)
Sk n be, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Avantor (1 mentions)
2 protocols using crl 2268
1
Neuroblastoma Cell Line Culture Protocol
2
Culturing Human Neuroblastoma Cell Lines
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The human NB cell lines KELLY (also known as N206; #92110411 from Sigma), SK-N-AS (called SKNAS in this study; CRL-213 from ATCC), SK-N-BE (clone SKNBE(2)C, called SKNBE in this study; CRL-2268 from ATCC), and SK-N-FI (called SKNFI in this study; from the Children’s Oncology Group) were cultured in RPMI 1640 (VWR), supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen), Penicillin (100 IU/mL) and Streptomycin (100 μg/mL) (30-002-CI, Corning). All cells were purchased from their respective suppliers within the last 2 years. The cells were maintained at 37 °C in a humidified atmosphere containing 5% CO2.
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