The eyes of the excised mice were fixed by TB-Fix [3.7% formaldehyde (Wako, 064-00406), 15% ethanol (Wako, 057-00456), 10% acetic acid (Wako, 017-00256)], as previously reported (Tokuda et al., 2018 (link)). Briefly, the eyes were kept overnight at 4°C in PBS containing 10% formaldehyde, 10% ethanol and 10% acetic acid. Fixed eyes were embedded with paraffin and sectioned to a thickness of 5 µm using a SM 200R microtome (Leica). Slices dried on glass slides were stained with H&E (Merck Millipore) after the paraffin was removed and dehydrated with alcohol and xylene. These sections were mounted using Marinol (Muto Pure Chemicals).
Sm 200r microtome
Manufactured by Leica
Sourced in Germany
The SM 200R microtome is a precise instrument designed for cutting thin sections of materials, particularly suitable for preparing samples for microscopic examination. It features a motorized cutting mechanism and advanced technical specifications to ensure accurate and reproducible results. The core function of the SM 200R is to enable the preparation of high-quality samples for various applications in the field of microscopy.
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Lab products found in correlation
2 protocols using sm 200r microtome
1
Histological Analysis of Mouse Eyes
2
Hematoxylin and Eosin Staining of Paraffin Sections
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Paraffin-embedded tissue sections (4 μm) prepared using an SM200R microtome (Leica, Wetzlar, Germany) were placed on slides pre-coated with poly-L-lysine followed by deparaffinization and rehydration. The sections were stained with hematoxylin and eosin followed by clearing with xylene for 5min x2 before mounting with DPX mounting medium and examination under an Olympus BX43 light microscope.
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