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3 protocols using phenol red free rpmi 1640 with l glutamine

1

Curcumin's Antiproliferative Effects on T47D Cells

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Curcumin (Sigma, Germany), MTT (Sigma, Germany), Leptin ELISA kit (Labor diagnostika nord gmbh & co. kg, Germany), Fetal bovine serum (Gibco, USA), Phenol-red free RPMI 1640 with L-glutamine (Gibco, USA), T47D cells (Pasteur Institute of Iran), Sodium bicarbonate (Merck, Germany), Penicillin (SERVA, Germany), Streptomycin (Merck, Germany), Amphotericin B (Merck, Germany), TRIZOL Reagent (Invitrogen, USA), First-Strand Synthesis kit (Fermentas, USA), Syber Green-I reagent (Fermentas, USA).
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2

Breast Cancer Cell Line Treatment

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Silibinin, Metformin and MTT powder were obtained from Sigma (Deisenhofen, Germany); T47D breast cancer cell line purchased from the Pasteur Institute of Iran, Tehran, Iran; fetal bovine serum (FBS) and phenol-red free RPMI 1640 with L glutamine were purchased from Gibco BRL (Life Technologies, Grand Island, NY, USA); Sodium bicarbonate and Streptomycin were purchased from Merck (Darmstadt, Germany); Penicillin G was purchased from SERVA (Heidelberg, Germany); TRIZOL reagent was purchased from Invitrogen (Eugene, OR, USA); First-Strand cDNA Synthesis kit was purchased from Fermentas (Hanover, MD, USA); and Syber Green-I reagent was purchased from Takara Bio (Otsu, Japan).
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3

Synthesis and Characterization of PEG-Sn(Oct)2-ε-CL Biomaterials

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PEG , stannous 2-ethylhexanoate (Sn(Oct)2) and "-Caprolactone ("-CL) were procured from Merc(Germany), Silibinin (SIL) [3,5,7-trihydroxy-4-oxobenzopyran-2-yl] benzodioxin], Curcumin (CUR), MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] were prepared from Sigma (St. Louis, MO) . Fetal bovine serum (Invitrogen, Carlsbad, CA), Phenol-red free RPMI 1640 with L-glutamine were procured from Gibco (Carlsbad, CA), Penicillin G and Streptomycin (Applichem, Darmstadt, Germany), TRIZOL Reagent (Invitrogen, USA), First-Strand Synthesis kit (Fermentas, Ontario, Canada), and Syber Green-I reagent were purchased from Takara (Tokyo, Japan).
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