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2 protocols using sodium triphosphate pentabasic tpp

1

AGP Chitosan Nanoparticle Formation and Characterization

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α1-acid glycoprotein (AGP) C.A.T. #(66455-27-4), PBS, high pure chitosan, and lipopolysaccharide (LPS) from E. coli 026:B6 were from Sigma (L8274-10MG). The QuantiPro TM BCA Assay kit was from Sigma Aldrich; St. Louis, MO, USA (QPBCA-1KA). High purity chitosan (deacetylation degree over 75–85% mol and viscosimetric molecular weight 50–190 kDa), sodium triphosphate pentabasic (TPP), 1 M hydrochloric acid (HCl), and sodium hydroxide (NaOH) were obtained from Sigma-Aldrich (Gillingham, UK). Milli-Q water was used in all synthetic experiments. Hyaluronic acid (HA; weight average molecular weight ≈200 kDa) was purchased from Medipol SA; Lausanne, Switzerland. Regenerated cellulose (RC) dialysis membrane (MWCO 10kDa) was obtained from Spectra Por, Spectrum Laboratories Inc.; Los Angeles, CA, USA.
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2

Chitosan Molecular Weight Characterization

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Chitosan differing in molecular weight, low molecular weight (LMW) (50-190 kDa, 75-85% deacetylated), medium molecular weight (MMW) (190-310 kDa, 75-85% deacetylated) and high molecular weight (HMW) ( 310-375 kDa, > 75% deacetylated) were purchased from Sigma Aldrich (Dorset, UK). Sodium triphosphate pentabasic (TPP), fluorescein sodium salt (FSS), and phosphotungstic acid (PTA) and acetic acid were also obtained from Sigma-Aldrich (Dorset, UK). Acacia, sodium hydroxide, phosphorous pentoxide, and anhydrous sodium carbonate were purchased from Fisher Scientific (UK). All reagents used were of analytical grade.
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