The antagonists or inhibitors involved in the NF-κB and MAPK signal pathways were introduced to verify the action of NP. The endocytosis inhibitors Pitstop2 (12.5 μM, Sigma) and Dynasore hydrate (12.5 μM, Sigma), the TLR4 antagonist LPS-RS (10 μg/ml; InvivoGen, San Diego, CA), the TLR1/TLR2 antagonist CU-CPT22 (20 μM, Millipore, Burlington, MA), the IL-1R antagonist (20 μM; Cayman Chemical Company), IKK-16 (20 μM; Cayman Chemical Company), the JNK inhibitor V (20 μM; Cayman Chemical Company), the ERK1/2 inhibitor U0126 (20 μM, Cell Signal Technology), and the p38 inhibitor SB203580 (20 μM; Enzo Life Sciences, Farmingdale, NY) were added to HLMEC cultures 1 h before NP exposure. The whole-cell lysates were harvested 8 h after NP stimulation and analyzed by Western blotting.
Il 1r antagonist
Manufactured by Cayman Chemical
Sourced in United States
IL-1R antagonist is a laboratory reagent that binds and blocks the interleukin-1 receptor (IL-1R), an important mediator of inflammation and immune response. Its core function is to inhibit the signaling pathway initiated by the binding of interleukin-1 to its receptor.
Automatically generated - may contain errors
Lab products found in correlation
Pitstop 2, by Merck Group (2 mentions)
Sb203580, by Enzo Life Sciences (2 mentions)
Lps rs, by InvivoGen (2 mentions)
Ikk 16, by Cayman Chemical (2 mentions)
Dynasore hydrate, by Merck Group (2 mentions)
Cu cpt22, by Merck Group (2 mentions)
Jnk inhibitor 5, by Cayman Chemical (2 mentions)
U0126, by Cell Signaling Technology (2 mentions)
Oregon green 488 gelatin, by Thermo Fisher Scientific (1 mentions)
Apo transferrin, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Insulin, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Cholera toxin, by Merck Group (1 mentions)
3 protocols using il 1r antagonist
1
Elucidating Signaling Pathways Modulating NP Effects
2
Cell Culture Reagents and Materials
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All reagents used in cell culture were purchased from Gibco BRL Co. (Rockville, MD, USA). Cholera toxin, hydrocortisone, insulin, apo-transferrin, T3, and dimethyl sulfoxide (DMSO) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Recombinant human interleukin 1 beta (IL-1β) was purchased from R&D Systems (Minneapolis, MN, USA). IL-1R antagonist was purchased from Cayman (Cayman Chemical, Ann Arbor, MI, USA). GM6001 was purchased from Calbiochem (La Jolla, CA, USA). Oregon Green 488 gelatin were purchased from Molecular Probes (Carlsbad, CA, USA).
3
Deciphering NF-κB and MAPK Signaling Pathways
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The antagonists or inhibitors involved in NF-κB and MAPK signal pathway were introduced to verify the action of NP. The endocytosis inhibitors Pitstop 2 (12.5 μM, Sigma) and Dynasore hydrate (12.5 μM, Sigma), the TLR4 antagonist LPS-RS (10 μg/mL, InVivoGen, San Diego, CA), the TLR1/TLR2 antagonist CU-CPT22 (20 μM, Millipore, Burlington, MA), the IL-1R antagonist (20 μM, Cayman Chemical Company), IKK-16 (20 μM, Cayman Chemical Company), the JNK inhibitor V (20 μM, Cayman Chemical Company), the ERK1/2 inhibitor U0126 (20 μM, Cell Signal Technology), and the p38 inhibitor SB203580 (20 μM, Enzo Life Sciences, Farmingdale, NY) were added into HLMEC cultures 1 h before NP exposure. The whole cell lysates were harvested 8 h after NP stimulation and analyzed by western blot.
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