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Apc conjugated f4 80 and epcam

Manufactured by BD

APC-conjugated F4/80 and EpCAM are laboratory reagents used in flow cytometry applications. F4/80 is a cell surface marker for macrophages, while EpCAM is a cell surface marker for epithelial cells. The APC (Allophycocyanin) fluorescent dye is conjugated to these antibodies, allowing for their detection and quantification in cell samples.

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2 protocols using apc conjugated f4 80 and epcam

1

Lung Leukocyte Phenotyping in Mice

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The phenotype of cells from mouse lungs was determined by incubating lung leukocytes with the indicated antibodies and CD16/32 to limit nonspecific binding. Leukocytes were stained at 4°C for 15 min in PBS containing 1%BSA and 0.01% sodium azide. Cells were stained with combinations of the following antibodies: FITC-conjugated I-Ab; PE-conjugated CD11c; PerCP-conjugated CD45; and APC-conjugated F4/80 and EpCAM from BD Biosciences. For intracellular IL-33 staining, cells were incubated with Cytofix/Cytoperm (BD Biosciences, San Diego, CA), washed in Permeabilization Buffer (BD Biosciences), and stained for 30 min with PE-conjugated IL-33 (R&D systems, Minneapolis, MN). Cells were washed and resuspended in 1% paraformaldehyde. Isotype controls were used. Data was acquired using BD Accuri™ C6 cytometer and analyzed using FCS Express 4.0 Software. For cell sorting experiments, F4/80+ leukocytes from the lungs of WT and CCR2−/− mice were isolated at day-7 p.i. using 5-laser FACS Aria II (BD Biosciences).
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2

Lung Leukocyte Phenotyping in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
The phenotype of cells from mouse lungs was determined by incubating lung leukocytes with the indicated antibodies and CD16/32 to limit nonspecific binding. Leukocytes were stained at 4°C for 15 min in PBS containing 1%BSA and 0.01% sodium azide. Cells were stained with combinations of the following antibodies: FITC-conjugated I-Ab; PE-conjugated CD11c; PerCP-conjugated CD45; and APC-conjugated F4/80 and EpCAM from BD Biosciences. For intracellular IL-33 staining, cells were incubated with Cytofix/Cytoperm (BD Biosciences, San Diego, CA), washed in Permeabilization Buffer (BD Biosciences), and stained for 30 min with PE-conjugated IL-33 (R&D systems, Minneapolis, MN). Cells were washed and resuspended in 1% paraformaldehyde. Isotype controls were used. Data was acquired using BD Accuri™ C6 cytometer and analyzed using FCS Express 4.0 Software. For cell sorting experiments, F4/80+ leukocytes from the lungs of WT and CCR2−/− mice were isolated at day-7 p.i. using 5-laser FACS Aria II (BD Biosciences).
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