To assess effects on neuronal survival, half turn SG explants were cultured as above for 72 h, except that the explants were grown on glass cover slips. The explants were fixed as above, treated with 0.5 % peroxide in methanol to block endogenous peroxidases, reacted with a mouse monoclonal antibody IgG against rat neurofilament 200 (Sigma- Aldrich), followed by a biotinylated secondary anti-mouse IgG, and developed by an avidin and DAB procedure (Vector Laboratories, Burlingame, CA, USA). The tissue was cleared with citrosol (Fischer Scientific, Waltham, MA, USA) to allow the visualization of the cell somas for the evaluation of neuronal survival. 36 SG explants were studied for each condition.
Citrosol
Manufactured by Thermo Fisher Scientific
Sourced in United States
Citrosol is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed for the purification and separation of chemical compounds or biological samples. The core function of Citrosol is to provide a reliable and efficient method for isolating and concentrating target analytes from complex matrices.
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Lab products found in correlation
2 protocols using citrosol
1
Assessing Neuronal Survival in SG Explants
2
Assessing Neuronal Survival in SG Explants
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To assess effects on neuronal survival, half turn SG explants were cultured as above for 72 hours, except that the explants were grown on glass cover slips. The explants were fixed as above, treated with 0.5% peroxide in methanol to block endogenous peroxidases, reacted with a mouse monoclonal antibody IgG against rat neurofilament 200 (Sigma-Aldrich), followed by a biotinylated secondary anti-mouse IgG, and developed by an avidin and DAB procedure (Vector Laboratories, Burlingame, CA). The tissue was cleared with citrosol (Fischer Scientific, Waltham, MA, USA) to allow visualization of the cell somas for evaluation of neuronal survival. 12 SG explants were studied per condition.
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