Analyst version 1

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Analyst version 1.4.2 is a laboratory instrument designed for analytical applications. It is capable of performing various analytical techniques, such as spectroscopy, chromatography, and mass spectrometry. The core function of the Analyst is to provide reliable and accurate data analysis for researchers and scientists in various fields of study.

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Lab products found in correlation

Api 5500q system, by Thermo Fisher Scientific (2 mentions) Sciex 5500 qtrap mass spectrometer, by AB Sciex (1 mentions) Agilent poroshell 120 ec c18 column, by Agilent Technologies (1 mentions) Msk caa 1, by Cambridge Isotopes (1 mentions) Sil 30ac autosampler, by Shimadzu (1 mentions) Lc 30ad pump, by Shimadzu (1 mentions)

Close spelling variants of this product

Analyst Software version 1.6 Analyst Software (version 1.4; Analyst 1.5 software

13 protocols using analyst version 1

Milvexian PK and Anticoagulation Assays

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Plasma samples were analyzed for milvexian concentration and milvexian protein binding using a validated liquid chromatography with tandem mass spectrometry (LC-MS/MS) assay at ICON Laboratory Services, Inc. (Whitesboro, NY, USA). Samples were analyzed using Analyst version 1.4.2 (Applied Biosystems, Framingham, MA, USA). LC-MS/MS assays had a lower limit of quantification of 1.0 ng/mL and an upper limit of quantification of 1000 ng/mL. Individual participant PK parameter values were derived by noncompartmental methods using a validated PK analysis program. The cumulative milvexian Urt was calculated as the summation of the product of the concentration of the analyte with the volume of urine collected over a collection interval. To determine CL_R, the cumulative amount of milvexian excreted in urine was divided by the plasma AUC over the same time interval, data permitting. The designated protein-binding blood sample was analyzed for milvexian protein binding by LC-MS/MS. Validated assays were performed at Labcorp Colorado Coagulation to measure aPTT and FXI clotting activity (Englewood, CO, USA).

Perera V., Abelian G., Li D., Wang Z., Zhang L., Lubin S., Bello A, & Murthy B. (2022). Single-Dose Pharmacokinetics of Milvexian in Participants with Normal Renal Function and Participants with Moderate or Severe Renal Impairment. Clinical Pharmacokinetics, 61(10), 1405-1416.

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Quantitative HPLC-MS/MS Analysis of Ketamine

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Ketamine quantification in Ketaset was carried out using a previously described high-performance liquid chromatography (HPLC) in tandem with mass spectrometry (MS/MS) method (Zanos et al., 2016 (link)). Briefly, the HPLC was accomplished using an Eclipse XDB-C18 guard column (4.6 mm × 12.5 mm) and a Varian Pursuit XRs 5 C18 analytical column (250 mm × 4.0 mm ID, 5 μm; Varian, Palo Alto, CA, USA). The mobile phase consisted of ammonium acetate [5 mM, pH 7.6] as component A and acetonitrile as component B. A linear gradient was run as follows: 0 min 20% B; 5 min 20% B; 15 min 80% B; 20 min 20% B at a flow rate of 0.4 mL/min. The total run time was 30 min per sample. For the samples, the calibration standards ranged from 7.875 to 250 ng/mL and were made in the according matrix. The quantitation of ketamine was accomplished by calculating area ratios using d₄-ketamine (10 μL of 100 ng/ml solution) as the internal standard. Samples were diluted as needed to be within the necessary linear range (1:10,000 and 1:100,000). The MS/MS analysis was performed using a triple quadrupole mass spectrometer model API 4000 system from Applied Biosystems/MDS Sciex equipped with Turbo Ion Spray^® (TIS; Applied Biosystems, Foster City, CA). The data were acquired and analyzed using Analyst version 1.4.2 (Applied Biosystems).

Brown K.A., Zanos P., Powels C.F., Fix C.J., Michaelides M., Pereira E.F., Moaddel R, & Gould T.D. (2022). Ketamine preservative benzethonium chloride potentiates hippocampal synaptic transmission and binds neurotransmitter receptors and transporters. Neuropharmacology, 225, 109403.

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Quadruplicate Protein Analysis

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All of the analyses were performed in quadruplicate and the results expressed as mg/100 g of protein. Statistical calculations were performed using Matlab R2009b (Natick, MA) while for mass spectrometry data, Analyst version 1.4.2 (Applied Biosystems, Carlsbad, CA) was used.

Troise A.D., Fiore A., Wiltafsky M, & Fogliano V. (2015). Quantification of Nε-(2-Furoylmethyl)-L-lysine (furosine), Nε-(Carboxymethyl)-L-lysine (CML), Nε-(Carboxyethyl)-L-lysine (CEL) and total lysine through stable isotope dilution assay and tandem mass spectrometry. Food chemistry, 188.

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Quantitative Analysis of Pharmaceuticals in Plasma

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We used 20 A ΜPLC- Q-trap 4500 MS (Shimadzu, Japan, SCIEX, United States) to analyze the four components of the dissolved solutions and rabbit plasma samples. TMP, FA, NK, and ISP were scanned with an electrospray ionization source (ESI) in positive ion detection mode. The multiple reaction monitoring (MRM) mode was applied for quantitative analysis. The instrument parameters included an ion spray voltage of 5.5 kV, capillary temperature of 500°C, GS1 of 60 psi, and GS2 of 50 psi. The MS parameters of the four analytes are shown in Table 2. Data analysis was conducted with Analyst version 1.6.2 (Applied Biosystems/MDS SCIEX).
Four analytes were separated on ACQMITY MPLC®BEH C₁₈ (1.7 μm, 2.1 × 100 mm) at 30°C with a flow rate of 0.3 ml/min. The mobile phase consisted of acetonitrile (A) and 0.1% formic acid water (B) with gradient elution. For quantitative determination of the four ingredients, the gradient elution program was as follows: 0.0–0.1 min, 30% A; 0.1–2.6 min, 30%–50% A; 2.6–3.0 min, 50%–61% A; 3.0–7.5 min, 61% A; 7.5–10.0 min, 61%–30% A; and 10.1 min, stop. The injection volume of samples was 2 μL.

Qu Z.H., Liu L., Zhang X.F., Guo D.Y., Zhai B.T., Zou J.B, & Shi Y.J. (2022). Exploring the Scientific Rationality of the Phenomenon of “Different Dosage Forms of the Same Prescription” of Chinese Proprietary Medicine Based on Biopharmaceutical Properties of Powder and Pill of Chuanxiong Chatiao Prescription. Frontiers in Pharmacology, 13, 893552.

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Analytical Software Protocol for Biomolecules

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The analytical data were processed by the software Analyst version 1.6.2 (Applied Biosystems, Foster City, CA). The data were also analysed in Lightsight version 2.3.0 (Applied Biosystems). ChemBioDraw Ultra version 13.0 (CambridgeSoft) was used to draw structures.

Connarn J.N., Luo R., Windak J., Zhang X., Babiskin A., Kelly M., Harrington G., Ellingrod V.L., Kamali M., McInnis M, & Sun D. (2016). Identification of non‐reported bupropion metabolites in human plasma. Biopharmaceutics & Drug Disposition, 37(9), 550-560.

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Gene Expression Analysis Protocol

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Data was obtained and analyzed using Analyst version 1.6.2 software, from Applied Biosystems. Values were expressed as mean ± SD for all data. P < 0.05 was considered statistically significant.

Li G., Zhao M., Su X., Song L, & Zhao L. (2018). Preparation and in vitro-in vivo evaluation of intestinal retention pellets of Berberine chloride to enhance hypoglycemic and lipid-lowing efficacy. Asian Journal of Pharmaceutical Sciences, 14(5), 559-568.

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Tandem Mass Spectrometry for Derivatized Amino Acids

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Tandem mass spectrometry (MS/MS) analysis was performed using a triple quadrupole mass spectrometer model API 5500Q system from Applied Biosystems/MDS Sciex equipped with Turbo V electrospray ionization source (TIS)^® (Applied Biosystems, Foster City, CA, USA). The data was acquired and analyzed using Analyst version 1.5.1 (Applied Biosystems). Positive electrospray ionization data were acquired using multiple reaction monitoring (MRM). The TIS instrumental source settings for temperature, curtain gas, ion source gas 1 (nebulizer), ion source gas 2 (turbo ion spray), collision energy, and ion spray voltage were 550 °C, 20 psi, 45 psi, 80 psi, 15 V, and 4,500 V, respectively. The TIS compound parameter settings for declustering potential, entrance potential, and collision cell exit potential were 80, 10, and 10 V, respectively. The standards were characterized using the following MRM ion transitions: D-Ser and L-Ser derivatization products (m/z 231.5 to 106.1) and D-Arg derivatization product (m/z 300.4 to 175.0).

Moaddel R., Luckenbaugh D.A., Xie Y., Villaseñor A., Brutsche N.E., Machado-Vieira R., Ramamoorthy A., Lorenzo M.P., Garcia A., Bernier M., Torjman M.C., Barbas C., Zarate CA J.r, & Wainer I.W. (2014). D-serine plasma concentration is a potential biomarker of (R,S)-ketamine antidepressant response in subjects with treatment-resistant depression. Psychopharmacology, 232(2), 399-409.

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Mass Spectrometry Analysis of Amino Acid Derivatives

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MS/MS analysis was performed using a triple quadrupole mass spectrometer model API 5500Q system from Applied
Biosystems/MDS Sciex equipped with Turbo V electrospray ionization source (TIS)^® (Applied Biosystems,
Foster City, CA, USA). The data was acquired and analyzed using Analyst version 1.5.1 (Applied Biosystems). Positive
electrospray ionization data were acquired using multiple reaction monitoring (MRM). The TIS instrumental source settings for
temperature, curtain gas, ion source gas 1 (nebulizer), ion source gas 2 (turbo ion spray), collision energy and ion spray
voltage were 550 °C, 20 psi, 45 psi, 80 psi, 15 V and 4500 V, respectively. The TIS compound parameter settings for
declustering potential, entrance potential, and collision cell exit potential were 80 V, 10 V, and 10 V, respectively. The
standards were characterized using the following MRM ion transitions: DSR derivatization product
(m/z 231.5 to 106.1) and D-Arg derivatization product
(m/z 300.4 to 175.0).

Pradhan B., Mitrev L., Moaddel R, & Wainer I.W. (2018). D-Serine is a potential biomarker for clinical response in treatment of post-traumatic stress disorder using (R,S)-ketamine infusion and TIMBER psychotherapy: A pilot study. Biochimica et biophysica acta. Proteins and proteomics, 1866(7), 831-839.

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Fatty Acid Quantification by LC-MS/MS

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Detailed methods are included in the Data S1. In brief, samples were extracted with chloroform/methanol, and the organic layer was isolated and subjected to solid phase extraction. Fatty acids were then eluted with chloroform. The eluate was reconstituted in a resuspension solvent containing internal standard (heptadecanoic acid) and used for LC‐MS/MS analysis. A concentrated stock solution was prepared containing five fatty acids: palmitic acid, stearic acid, arachidic acid, behenic acid, and lignoceric acid (250 μg/mL each). Serial dilutions of the stock solution were used to prepare the samples for the calibration curve with concentrations ranging from 10 μg/mL to 0.3125 μg/mL. Data were acquired and analyzed using Analyst version 1.5.1 (Applied Biosystems).

Bhargava P., Fitzgerald K.C., Venkata S.L., Smith M.D., Kornberg M.D., Mowry E.M., Haughey N.J, & Calabresi P.A. (2018). Dimethyl fumarate treatment induces lipid metabolism alterations that are linked to immunological changes. Annals of Clinical and Translational Neurology, 6(1), 33-45.

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Quantification of Serum Beta-Hydroxybutyrate

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The serum concentration of BHB was quantified using an LC-MS/MS system comprising a 1,260 Infinity II Series high-performance liquid chromatography (HPLC) (Agilent, Santa Clara, CA, USA) coupled with a Sciex 5,500 QTRAP mass spectrometer (AB Sciex, Foster City, CA, USA). MS parameters were set as previously described (18 (link)). BHB standards, serum samples, and quality controls were, respectively, mixed with the isotopically labeled internal standard (BHB-D4) solution and proteins were precipitated with methanol containing 0.5% formic acid. After vortexing and centrifuging, the metabolites were separated by a Kinetex™ hydrophilic interaction LC column (2.6 μm, 150 mm × 2.1 mm, Phenomenex, Torrance, CA, USA) and eluted with a mobile phase of 75% methanol, containing 5 mmol/L ammonium formate, and 0.05% formic acid at a flow rate of 0.3 ml/min. BHB and internal standard were detected with positive electrospary ionization in multiple reaction monitor mode using characteristic precursor–product ion transitions of m/z 103.0 → 59.0 and m/z 107.0 → 59.0, respectively. The concentration of BHB was calculated using standard curves. The analytical coefficients of variation for BHB measurements were below 3%. Applied Biosystems Analyst version 1.6.2 software was used for system control, data collection, and processing.

Mu H., Yang R., Wang S., Zhang W., Wang X., Li H., Dong J., Chen W., Yu X, & Ji F. (2022). Association of Serum β-Hydroxybutyrate and Coronary Artery Disease in an Urban Chinese Population. Frontiers in Nutrition, 9, 828824.

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