Mouse anti app

The following antibodies were used: rabbit anti-RPN1 (Abcam, ab198508), mouse anti-GAPDH (Merck Millipore, MAB374), mouse anti-Myc (Millipore, 05-724), mouse anti-FLAG (Sigma, F1804), rabbit anti-GM130 (Cell Signaling Technology, 12480), rabbit anticalnexin (Abcam, ab22595), mouse anti-APP (Millipore, MAB348), mouse anti-β-catenin (BD bioscience, 610154), mouse anti-LgBiT (Promega N7100), horseradish peroxidase (HRP)–conjugated antimouse IgG (GE Healthcare, NA931V), HRP-conjugated antisheep IgG (Abcam, ab6900), HRP-conjugated anti-rabbit IgG (GE Healthcare, NA934V), Alexa546-conjugated antisheep IgG (Invitrogen, A21098), Alexa546-conjugated antimouse IgG (Invitrogen, A10036), and Alexa488-conjugated anti-rabbit IgG (Invitrogen, A21206). Mouse anti-FUT8 (15C6) was kindly provided by Dr Eiji Miyoshi (Osaka University). Biotinylated PhoSL (26 (link)) was kindly provided by J-Oil Mils. MG132 was purchased from Merck and dissolved in dimethyl sulfoxide. CQ was purchased from Wako and was dissolved in water. CHX was purchased from Wako and was dissolved in dimethyl sulfoxide.

Rabbit anti-phospho ERK (T202/Y204; #4370), anti-ERK (#9102), anti-phospho Akt (S473; #9271), anti-Akt (#9272), anti-phospho EGF receptor (EGFR) (Y1068; #3777), and anti-EGFR (#4267) were from Cell Signaling Technology (Danvers, MA, USA). Mouse anti-EpCAM (#sc-25308) was from Santa Cruz Biotechnologies (Santa Cruz, CA, USA), and mouse anti-pan actin (#MA5-11869) was from Thermo Scientific (Lafayette, CO, USA). Mouse anti-APP (#MAB348) and rabbit anti-ADAM17 (#AB19027) were from Millipore (Billerica, MA, USA). Rabbit anti-ADAM17 (#ab39162), rabbit anti-ADAM10 (#ab1997), goat anti-Met receptor (#ab10728), and mouse anti-glucose-6-phosphate (GPI) (#ab66340) antibodies were from Abcam (Cambridge, MA, USA). Rabbit anti-DDR1 (#10730) was from Sino Biological Inc. (Beijing, PR China), rabbit anti-GAPDH (#2275-PC-1) was from Trevigen (Gaithersburg, MD, USA), and mouse anti-HER2 (#MU-134-UC) was from BioGenex (San Ramon, CA, USA). Mouse anti-EphB4 (#AF446) was from R&D Systems (Minneapolis, MN, USA). Fluorochrome-conjugated antibodies were from Molecular Probes (Life Technologies, Grand Island, NY, USA).