According to our previous research (Tang et al., 2022 (link)), RRTP is extracted from RRT fruit and preserved in microbiology and biochemical pharmaceutical engineering research center of Guizhou Medical University. Lipopolysaccharide (LPS, Escherichia coli serotype 055:B5) was purchased from Sigma-Aldrich. Acetonitrile and methanol (UPLC-grade) were collected from Merck.
Methanol uplc grade
Manufactured by Merck Group
Sourced in Germany
Methanol (UPLC-grade) is a high-purity solvent used in ultra-performance liquid chromatography (UPLC) applications. It is a colorless, volatile, and flammable liquid with a characteristic odor. The UPLC-grade designation indicates that the methanol meets the stringent purity and quality requirements for UPLC instrumentation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Merck Group (2 mentions)
Lipopolysaccharide lps escherichia coli serotype 055 b5, by Merck Group (1 mentions)
Millipore q sp reagent water system, by Merck Group (1 mentions)
Formic acid, by Tianjin Kemiou Chemical Reagent (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Hplc grade methanol, by Tedia (1 mentions)
Trifluoroacetic acid, by Sinopharm (1 mentions)
4 protocols using methanol uplc grade
1
RRTP Extraction and Characterization
2
Aflatoxin B1 Quantification in Peanut Oil
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AFB1 (2,3,6α,9α-tetrahydro-4-methoxycyclopenta ［c］ furo ［3 ,2 :4,5］ furo ［2,3-h］ ［ 1］ benzopyran-1,11-dione; C 17 H 12 O 6 ) standard with purity above 99％ was purchased from Romer Labs Inc. (Singapore) . Ultrapure water was produced using a Millipore-Q SP reagent water system (Millipore, Bedford, MA, USA) . Acetonitrile (UPLC grade) , methanol (UPLC grade) , formic acid (UPLC grade) , and the other chemicals were obtained from Merck (Merck KGaA, Germany) . Ten peanut oil samples (2.0 L) were obtained from the Guangxi Youqing Miyi Technology Co., Ltd. (Guangxi, China) . These samples were labeled as S1-S10 (S1: Myanmar; S2: Hubei, China; S3: Henan, China; S4: Quang Ninh, Vietnam; S5: Lang Son, Vietnam; S6: Henan, China; S7: Cao Bang, Vietnam; S8: Henan, China; S9: Hubei, China; S10: South Africa) .
3
Multicomponent Herbal Formula for Therapeutic Research
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KLX was composed of Astragali radix (Dried root of Astragalus mongholicus Bunge), Pseudostellariae radix (Dried root tuber of Pseudostellaria heterophylla (Miq.) Pax), Ligustri lucidi fructus (Dried mature fruits of Ligustrum lucidum W.T.Aiton), Lycii fructus (Dried mature fruits of Lycium barbarum L.), Rhei radix et rhizome (Dried root and rhizome of Rheum officinale Baill.), Hirudo (Dried body of Hirudo nipponica Whitman). It was provided by Chengdu Kanghong Pharmaceutical Co., Ltd., Chengdu, China. UPLC-grade methanol and acetonitrile were purchased from Merck KGaA, Darmstadt, Germany. Distilled water was obtained from Watsons Water, Guangzhou, China. Formic acid was purchased from Tianjin Kemiou Chemical Reagent Co., Ltd., Tianjin, China.
4
Nodularin Quantification in Bacterial Strains
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The previously obtained strains Sphingopyxis sp. m6, recombinant mlrA-HMBP-pET28a/BL21, and HMBP-pET28a/BL21 were investigated in this study [37 (link)]. Standard NOD (C41H60N8O10, ≥95% purity) purchased from APExBIO (Houston, USA) was dissolved in methanol at a concentration of 100 mg/L and stored at −20 °C. The mineral salt medium (MSM) was used as an essential component of the experimental systems. The real-time quantitative polymerase chain reaction (RT-qPCR) kit was supplied by TaKaRa (Kusatsu, Japan). Trifluoroacetic acid (Sinopharm Chemical Reagent Co., Ltd. Shanghai, China) and HPLC-grade methanol (TEDIA Co., Fairfield, OH, USA) were applied to the liquid phase. UPLC grade methanol (Merck KGaA, Darmstadt, Germany) and formic acid (Fisher Scientific, Shanghai, China) were used for mass spectrometry.
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