Blood samples (0.8 mL) were extracted at the endpoints of experiments and maintained at RT for 30 min, followed by centrifugation at 3000 rpm for 15 min at 4 °C to facilitate supernatant collection. After treatment, the AC16 cells were collected via centrifugation. The quantification of MDA (malonaldehyde; E-EL-0060c, Elabscience, China), CK-MB (creatine kinase isoenzyme MB; E-E-M0355c, Elabscience, China), GSH (glutathione; E-BC-K030-M, Elabscience, China), ATP (E-BC-K157-M, Elabscience, China), and LDH (lactate dehydrogenase; E-BC-K046-M, Elabscience, China) in mouse serum or AC16 cells of different groups was performed using an ELISA kit or activity assay kit following the manufacturers’ protocols. MitoTEMPO (Aladdin, 1,334,850–99-5, China) was used to verify whether the mitochondrial targeted ROS inhibitor causes damage to cardiomyocytes.
E bc k046 m
Manufactured by Elabscience
Sourced in China
E-BC-K046-M is a lab equipment product manufactured by Elabscience. It serves as a centrifuge for the separation of substances with different densities from a given sample.
Automatically generated - may contain errors
Lab products found in correlation
E el 0060c, by Elabscience (1 mentions)
E bc k157 m, by Elabscience (1 mentions)
E bc k030 m, by Elabscience (1 mentions)
E bc k020, by Elabscience (1 mentions)
E el r0012, by Elabscience (1 mentions)
E el r0506, by Elabscience (1 mentions)
E bc k025 s, by Elabscience (1 mentions)
Ebc k235, by Elabscience (1 mentions)
2 protocols using e bc k046 m
1
Quantifying Cardiac Biomarkers in Cells
2
Quantification of Hepatic Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Utilizing the guidelines provided by the manufacturer, the Elisa Kit was employed to quantify many parameters. The measurements included MDA levels (Elabscience, E-BC-K025-S), hepatic SOD levels (Elabscience, E-BC-K020), CAT levels (Elabscience, E-BC-K031), hepatic LDH levels (E-BC-K046-M), hepatic ACP levels (E-BC-K010-M), hepatic AST levels (Elabscience, E-BC-K236), hepatic ALT levels (Elabscience, E-BC-K235), hepatic IL-1β levels (E-EL-R0012), and hepatic CRP levels (Elabscience, E-EL-R0506) [14 ,18 (link)].
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