Anti nitrotyrosine nt polyclonal antibody

AA was obtained from Calbiochem (San Diego, CA, USA). Antibodies against procaspase-3, cleaved caspase-3, poly (ADP-ribose) polymerase (PARP), Bcl-2, lamin A/C, ERK1/2, phospho-ERK1/2, and NAD(P)H:quinone oxidoreductase 1 (NQO1) along with horseradish peroxidase-conjugated goat anti-mouse antibodies were provided by Cell Signaling Technology (Beverly, MA, USA). Anti-Nrf2 and anti-cleaved PARP antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The anti-phospho-Nrf2, anti-glutamate-cysteine ligase catalytic subunit (GCLC), and anti-4-hydroxynonenal (4-HNE) polyclonal antibodies were purchased from Abcam (Cambridge, MA, USA). The Fugene® HD and luciferase assay kit were obtained from Promega (Madison, WI, USA). Anti-nitrotyrosine (NT) polyclonal antibody was purchased from Millipore Corporation (Bedford, MA, USA). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), rhodamine 123 (Rh123), 2′,7′-dichlorofluorescein diacetate (DCFH-DA), silymarin (SIL), anti-β-actin antibody, and other reagents were supplied by Sigma-Aldrich (St. Louis, MO, USA).

AA and compound C were obtained from Calbiochem (San Diego, CA, USA). Anti-poly [ADP-ribose] polymerase (PARP), anti-procaspase-3, anti-phospho-AMPKα, and horseradish peroxidase- (HRP-) conjugated goat anti-mouse antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA). HRP-conjugated goat anti-rabbit antibody was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Dimethyl sulfoxide (DMSO) was obtained from Junsei Chemical (Tokyo, Japan). Anti-4-hydroxynonenal (HNE) polyclonal antibody was from Abcam (Cambridge, UK) and anti-nitrotyrosine (NT) polyclonal antibody was obtained from Millipore (Temecula, CA, USA). The VECTASTAIN Elite ABC HRP Kit and Peroxidase Substrate Kit were supplied from Vector Labs (Burlingame, CA, USA). Nitrilotriacetic acid, ferric nitrate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′7′-difluorescin diacetate (DCFH-DA), rhodamine (Rh)123, anti-β-actin antibody, acacetin, apigenin, luteolin, and other chemicals were obtained from Sigma-Aldrich (St. Louis, MO, USA).